Table of Contents
What media is used to grow Rhizopus?
Growth of Rhizopus stolonifer on different media, after 24 h: A) potato-dextrose-agar (PDA), B) malt-agar (MA), C) Czapeck (CZ), and after 48h: D) PDA, E) MA, F) CZ. Rhizopus stolonifer commonly causes postharvest diseases on many fruits and vegetables.
What medium is commonly used when culturing molds on agar plates?
The media most commonly used are nutrient agar (bacteria), potato dextrose agar (fungi), and Sabouraud dextrose agar (fungi).
What are the materials required to observe spores of Rhizopus?
Materials required:
- 5 slices of bread.
- 5 transparent sealable bags.
- Gloves.
- mask.
- Sticky labels.
- marker.
- Magnifying glass.
- 5 – 7 cotton swabs.
How can you prepare a temporary slide of Rhizopus explain?
In this article, we will learn how to prepare a temporary mount of Rhizopus….Prepare the Rhizopus
- Take a slice of soft bread and leave it in open air for about 60 min, place the bread within a plastic bag.
- Spray some water over the bread to have dampness then seal the bag.
- Store this bag in a dark and warm place.
How do you incubate fungal plates?
(a) First incubate at 20-25°C temperature for 72 hours for fungal growth and then same plates transferred to 30-35°C for further 48 hours for bacterial growth. (b) First incubate at 30-35°C temperature for 48 hours for bacterial growth and then same plates transferred to 20-25°C for further 72 hours for fungal growth.
How do you perform a fungal culture test?
Blood Test
- Used to detect the presence of fungi in the blood. Blood tests are often used to diagnose more serious fungal infections.
- Test procedure: A health care professional will need a blood sample. The sample is most often taken from a vein in your arm.
What is direct plating method?
MOSTLY UNAMBIGUOUSLY USED TERM. Means of phage growth and enumeration that involves both phage and bacterial application to the surface of an agar slab as found within a Petri dish.
How do you grow mold on agar plates?
Move the dish to the refrigerator until you need them, and then introduce the bacteria by rubbing a swab or dropping liquid onto the agar. Then, use a piece of tape to label and seal the dish, and place it in a dark, warm place for 4-6 days to grow the bacteria.
What procedure did you followed to observe Rhizopus in laboratory?
Take the cotton swabs and rub it in some dust, then transfer the dust from cotton swab over small pieces of bread. Add some fruit juice to the bread pieces and place them in transparent bags and leave them for a few days. Observe the pieces carefully at a fixed time each day under a microscope.
What procedure do you follow to observe Rhizopus?
I) To observe Rhizopus he has to do following preparatory activities:
- He has to grow Rhizopus or common mould on his own in a controlled environment.
- Take a bread slice, leave in open air for about an hour, place the bread in plastic bag.
- Sprinkle water over it to have dampness then seal the bag, leaving some air.
What are the material required to prepare a slide of Rhizopus in your laboratory?
Materials Required: Mould sample, plain glass slide, coverslip, water, disposable gloves. Place a drop of water in the centre of the slide. Using a toothpick, scrape very little of the mould and place it on the drop of water.
What are the materials required to observe spore of Rhizopus?
Why do you incubate plates upside down?
Petri dishes need to be incubated upside-down to lessen contamination risks from airborne particles landing on them and to prevent the accumulation of water condensation that could disturb or compromise a culture.
What swab is used for fungal culture?
Swabs: Throat, nose, nasopharynx, and ear swabs are acceptable; material from the ear is better than a swab. Urine: Clean catch midstream sample in sterile container.
What is direct plating in microbiology?
How to prepare rhizobium bacterial culture?
Preparation of Rhizobium Bacterial Culture: A vigorously growing leguminous plant that has been grown for the time on the field is carefully uprooted. The root is carefully washed with sterilized water. The nodules are then carefully detached from the roots by cutting a portion of the root on either side of it.
What are the conditions required for Rhizopus stolonifer to grow?
Wet weather and the high temperatures during summer are very favourable for Rhizopus stolonifer development. Thus, it thrives at temperatures of around 23-27°C and high humidity. Thus, it thrives at temperatures of around 23-27°C and high humidity.
What is the mode of reproduction of Rhizopus?
Under this mode of reproduction, small fragments are formed on the body surface of rhizopus and due to accidental breakage, the stolon may break up into two or more than two small units and each unit or part is capable of growing as a mother mycelium. 2. Asexual Mode of Reproduction
What are the different types of hyphae in Rhizopus microsporus?
The branched hyphae are of three types: stolons, pigmented rhizoids, and unbranched sporangiophores. Figure: Macroscopic and microscopic morphology of Rhizopus microsporus CBS 700.68.