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Can you miniprep yeast?
The Zymoprep Yeast Plasmid Miniprep II is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution.
What is miniprep kit?
The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications, including fluorescent and radioactive sequencing and cloning. Even higher yields (up to 30 μg) can be achieved using the High-Yield Supplementary Protocol.
How do you isolate a plasmid from yeast?
Plasmid Isolation from Yeast using Qiagen “QiaPrep” Kit
- Grow culture to saturation in 4 ml selective media.
- Spin 1.5 ml for 10 min in microfuge tube, and remove supe.
- Add another 1.5ml of culture to pellet, spin, and remove supe (optional)
- Resuspend in 500 ul sterile dd H2O.
- Spin 10 min, remove supe.
How much DNA does a miniprep have?
The GeneJET Plasmid Miniprep Kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure utilizing a silica-based membrane within the spin column.
How much does miniprep yield?
For mini preps, we recommend that you harvest 1-5 ml of overnight bacterial culture. For maxi preps, if the plasmid is high-copy plasmid, we recommend using 100-150 ml of overnight bacterial culture; if the plasmid is low-copy plasmid, we recommend using 300-500 ml of overnight bacterial culture.
Is it okay to vortex plasmid DNA?
Vortexing and pipetting your plasmid — take it easy! Nicked or linear DNA may occur due to mechanical shearing of DNA if preps are vortexed or shaken too vigorously during isolation of the plasmid. So take it very easy; mix gently, don’t vortex and pipette softly and sparingly.
Can you freeze bacteria before miniprep?
I did mini prep on bacterial pellets that were frozen for a whole year and it worked fine.
How much DNA can you get from miniprep?
What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit? The average DNA concentration obtained with the DirectPrep 96 Miniprep Kit is 66 ng/ul (typically >50 ng/ul) in an eluate volume of 50 ul. Up to 4 ug DNA total can be purified from 1.3 ml of LB culture medium.