Table of Contents
What is the purpose of a macromolecule lab?
PURPOSE: To use indicators to test for the presence of lipids, carbohydrates, and proteins in various foods.
What macromolecule does Benedict’s test for?
simple carbohydrates
Benedict’s Test is used to test for simple carbohydrates. The Benedict’s test identifies reducing sugars (monosaccharide’s and some disaccharides), which have free ketone or aldehyde functional groups.
What macromolecule is Benedict’s reagent testing for?
One test for the presence of many simple carbohydrates is to use Benedict’s reagent. It turns from turquoise to yellow or orange when it reacts with reducing sugars. These are simple carbohydrates with unbound aldehyde or ketone groups.
What is the importance of macromolecules assays?
Macromolecule assays are a useful starting point for students to design additional experiments, because such assays are easy to carry out and generate reliable results.
What are indicators for macromolecules?
1.1 Aim
| Indicator | Macromolecule | Positive test |
|---|---|---|
| Iodine solution | Complex carbohydrate | Black |
| Iodine | Simple sugars | Colourless |
| Sudan III | Lipid | Orange |
| Biuret solution | Protein | Violet |
How do you test for carbohydrates proteins and lipids?
Add Biuret solution A to a solution of the food being tested and mix carefully. Then trickle a little Biuret solution B down the side of the tube. Look for a purple colouration where the solutions meet. Biuret reagent is sometimes available as a single solution .
What macromolecule does biuret test for?
Proteins
Test #4: Testing for Proteins Biuret is a reagent that will change color from blue to violet when it detects peptide bonds between amino acids of a protein.
What will happen to the color of each macromolecule that show positive test?
We say that an indicator test is positive when the color change indicates that the macromolecules is present and negative if there is no color change indicating the lack of the macromolecule.
What is a macromolecule simple definition?
: a very large molecule (as of a protein or rubber)