Table of Contents
Do you add loading dye to DNA ladder?
The ladder is diluted to a 1:4 solution in water for use (3 parts water for 1 part ladder). To make 100 µl, 75 µl of water are combined with 25 µl of the DNA ladder. Then, 20 µl of 6X loading dye is added and the solution is split into 60 µl aliquots (0.5 ml microcentrifuge tubes) and stored at -20 C.
What does KB mean in DNA ladder?
10.0 kilobases
The digested DNA includes fragments ranging from 0.5-10.0 kilobases (kb). The 3.0 kb fragment has increased intensity to serve as a reference band. The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
How does the ladder allow us to determine the size of DNA?
When run alongside an unknown PCR product in an agarose gel, the ladder allows you to estimate the size of the unknown fragment by comparing it to the closest band in the ladder lane, like so: Ladder is also run alongside RFLP products to help estimate the size of the restriction fragments.
How do you determine the size of DNA fragments?
To calculate the fragment size we simply need to subtract the bp difference between the two REs: 5198 bp – 5070 bp = 128 bp. DNA fragment 2 – a large fragment (and our desired DNA fragment as it contains all the features of the plasmid.
What does 6X dye mean?
The 6X Loading Dye (3 colors) is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three tracking dyes (bromophenol blue, xylene cyanol FF, and orange G) for visually tracking the DNA migration during the electrophoresis process.
How do you dilute 6X to 1X?
6X Universal / Glow Dyes Dilute one part 6X Dye solution into five parts of sample solution to give a final concentration of 1X Dye solution. The sample is then ready to load to a gel.
What is Thermo Scientific generuler DNA ladder?
Description Thermo Scientific GeneRuler 1 kb Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 75 bp to 20,000 bp on agarose gels. The DNA ladder consists of 15 DNA fragments and is provided with 6X TriTrack DNA Loading Dye. Highlights of 1 kb Plus DNA Ladder
What is generuler 1 KB plus DNA ladder?
Thermo Scientific GeneRuler 1 kb Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 75 bp to 20,000 bp on agarose gels. The DNA ladder consists of 15 DNA fragments and is provided with 6X TriTrack DNA Loading Dye. Highlights of 1 kb Plus DNA Ladder
What is generuler 1 KB plus?
Thermo Scientific GeneRuler 1 kb Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 75 bp to 20,000 bp on agarose gels. The DNA ladder consists of 15 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.Highlights of 1 kb Plus
What is Thermo Scientific generuler TriTrack DNA loading dye?
In April 2018 this product was upgraded to include TriTrack DNA Loading Dye for better visualization. Thermo Scientific GeneRuler 1 kb DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 250 bp to 10,000 bp on agarose gels.