What does flap endonuclease do?
Flap endonuclease 1 (FEN1) is an enzyme involved in the base excision repair pathway and counteracts replication stress, thus blocking the mechanism by which many drugs kill tumor cells.
What is 5 endonuclease?
The 5′ nuclease cleaves bifurcated DNAs in a structure-specific manner during removal of primers or damaged DNA, so its activity is likely to be coordinated with the polymerizing activity of the enzyme.
What are DNA flaps?
DNA flaps function as intermediates in DNA metabolism. 3′-Flaps are generated as intermediates for homologous recombination. 5′-flaps, on the other hand, arise as necessary but dangerous DNA structures during DNA replication and DNA damage response.
What is the meaning of flap in biology?
Flap. Anything broad and limber that hangs loose, or that is attached by one side or end and is easily moved; as, the flap of a garment. A cartilaginous flap upon the opening of the larynx. (
What is a 5 exonuclease?
T5 Exonuclease degrades DNA in the 5´ to 3´ direction (1). T5 Exonuclease is able to initiate nucleotide removal from the 5´ termini or at gaps and nicks of linear or circular dsDNA (1). However, the enzyme does not degrade supercoiled dsDNA (2). T5 Exonuclease also has ssDNA endonuclease activity.
What is the role of FEN1 in DNA replication?
FEN1 maintains stable telomeres by facilitating replication through the G-rich lagging strand telomere, thereby ensuring high fidelity telomere replication.
What is flap medicine?
(… flap) A type of surgery used to rebuild the shape of the breast after a mastectomy.
What is 3 ‘-> 5 exonuclease activity?
The 3′–>5′ exonuclease activity intrinsic to several DNA polymerases plays a primary role in genetic stability; it acts as a first line of defense in correcting DNA polymerase errors. A mismatched basepair at the primer terminus is the preferred substrate for the exonuclease activity over a correct basepair.