What is FAM in PCR?
FAM is used in the preparation of fluorescein-labeled oligonucleotide probes for the detection of the presence of the complementary nucleic acids or primers for polymerase chain reaction. Oligonucleotides labeled with fluorescein at one of the termini and with a quencher at the other can serve as molecular beacons.
What Colour is Vic dye?
green-yellow
VIC is a fluorescent dye that was originally developed by Applied Biosystems, but is now proprietary to Life Technologies. VIC has an absorbance maximum of 538 nm and an emission maximum of 554 nm, thus emitting in the green-yellow part of the visible spectrum.
What color is FAM and HEX?
Four differently colored fluorophores (FAM: green; HEX: orange; ROX: red; and Cy5: blue) and one universal quencher (DABCYL: black) are used to depict the principal of MCPC.
Is FAM blue or green?
Sequence primers labeled with blue (6-FAM) or green (HEX) fluorescent…
What color is FAM and Hex?
What is Rox in PCR?
ROX dye is an inert fluorescent dye that can be added as one of the components in a qPCR master mix. Unlike reporter dyes such as SYBR™ Green or FAM™ dye, the fluorescence of ROX dye is not affected by amplification of the PCR product.
What is FITC stands for?
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure.
Why is FITC used?
FITC is used for labeling of different biomolecules, e.g. immunoglobulins, lectins and other proteins, peptides, nucleic acids, nucleotides; oligo-and polysaccha- rides.
What is probe and primer?
A primer or probe is the key factor for direct detection and identification of a given target sequence via, e.g. fluorescence by base pairing, called hybridisation. Hybridisation acts as starting point for consecutive reactions like sequencing or PCR.
What is Rox reference dye?
ROX passive reference dye is an inert additive that provides a constant fluorescent signal for sample normalization throughout the real-time qPCR assay. Related Topics: qPCR Instrumentation, qPCR/Real-Time PCR Reagents, and qPCR Assay Design and Optimization.
What is the full form of FAM?
Fam or FAM is a colloquial term for ‘family and friend’ or an acronym of ‘friend and mate’ especially for intimate friends.
Is FAM same as FITC?
FITC and FAM are both derivatives of fluorescein. FITC is fluorescein with an isothiocyanate reactive group added, while 6-FAM is 6-carboxyfluorescein. FITC and 6-FAM have identical spectral characteristics. In experiments, 6-FAM performs exactly like FITC.
What is a nucleotide in genetics?
Genetics Review. A nucleotide is one of the structural components, or building blocks, of DNA and RNA. A nucleotide consists of a base (one of four chemicals: adenine, thymine, guanine, and cytosine) plus a molecule of sugar and one of phosphoric acid. More: C, T, and U are called pyrimidines and each has a single nitrogen-containing ring.
What are the subunits of nucleotides?
Nucleotides are composed of three subunit molecules: a nucleobase, a five-carbon sugar ( ribose or deoxyribose ), and a phosphate group consisting of one to three phosphates. The four nucleobases in DNA are guanine, adenine, cytosine and thymine; in RNA, uracil is used in place of thymine.
What is the meaning of nucleoside?
: any of several compounds that consist of a ribose or deoxyribose sugar joined to a purine or pyrimidine base and to a phosphate group and that are the basic structural units of nucleic acids (such as RNA and DNA) — compare nucleoside.
What is a nucleotide (nt)?
Nucleotide (abbreviated “nt”) is a common unit of length for single-stranded nucleic acids, similar to how base pair is a unit of length for double-stranded nucleic acids. The IUPAC has designated the symbols for nucleotides. Apart from the five (A, G, C, T/U) bases, often degenerate bases are used especially for designing PCR primers.