What is HPLC in simple terms?
High Performance Liquid Chromatography (HPLC) is a process of separating components in a liquid mixture. A liquid sample is injected into a stream of solvent (mobile phase) flowing through a column packed with a separation medium (stationary phase).
What is HPLC best used for?
High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture.
What is HPLC in organic chemistry?
High-performance liquid chromatography (or high-pressure liquid chromatography, HPLC) is a chromatographic technique that can separate a mixture of compounds and is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of the mixture.
How many phases are used in HPLC?
There are two phases for HPLC: the mobile phase and the stationary phase. The mobile phase is the liquid that dissolves the target compound.
Which column is used in HPLC?
The reversed-phase HPLC column is the most versatile and commonly used column type and can be used for a wide range of different types of analytes. Normal-phase HPLC columns have polar packing. The mobile phase is nonpolar and therefore usually an organic solvent such as hexane or methylene chloride.
What solvent is used in HPLC?
Non-polar solvents like chloroform, cyclohexane, benzene, carbon tetrachloride etc. are used.
What does HPLC stand for in chromatography?
With HPLC, HPLC stands for high-performance liquid chromatography, formerly known as high-pressure liquid chromatography. This is essentially the same as the basic column chromatography that you see there in yellow.
Is Khan Academy free for teachers?
Created by experts, Khan Academy’s library of trusted, standards-aligned practice and lessons covers math K-12 through early college, grammar, science, history, AP®, SAT®, and more. It’s all free for learners and teachers.
What is personalized learning in Khan Academy?
Personalized learning Students practice at their own pace, first filling in gaps in their understanding and then accelerating their learning. Created by experts, Khan Academy’s library of trusted, standards-aligned practice and lessons covers math K-12 through early college, grammar, science, history, AP®, SAT®, and more.