Table of Contents
What is the transport media for Neisseria gonorrhoeae?
The survival of N. gonorrhoeae present in clinical speciments when placed in Amies charcoal transport medium was compared to their survival when inoculated into Jembec chambers containing either modified Thayer-Martin medium (MTM) or modified New York City transport medium (MNYC).
Which selective media is used to isolate Neisseria gonorrhoeae?
GC-Lect, a new selective medium for the isolation of Neisseria gonorrhoeae which contains five antimicrobial agents, was evaluated with stock cultures and with 500 clinical specimens.
What agar does gonorrhea grow on?
N. gonorrhoeae is usually isolated on Thayer–Martin agar (or VPN) agar in an atmosphere enriched with 3-7% carbon dioxide. Thayer–Martin agar is a chocolate agar plate (heated blood agar) containing nutrients and antimicrobials (vancomycin, colistin, nystatin, and trimethoprim).
What is Cary Blair transport medium?
Remel Cary Blair Transport Medium is a semisolid medium recommended for use in the transportation and preservation of clinical specimens, primarily stool and rectal swabs.
What is Amies transport medium?
Amies is an improved transport medium containing charcoal to prolong the viability of pathogenic organisms. It is a semi-solid medium recommended for qualitative procedures for transporting clinical swab specimens to the laboratory.
Can Neisseria gonorrhoeae grow on MacConkey agar?
Note: Neisseria does not grow on MacConkey. Note: Growth, but no fermentation of lactose. Colorless colonies, medium is slightly yellow due to the increased pH resulting from bacterial digestion of peptone in the medium. Note: Gram-positives do not grow on MacConkey.
What is the difference between chocolate agar and Thayer Martin Agar?
Chocolate Agar is a non-selective medium for the cultivation of fastidious bacteria, especially Neisseria and Haemophilus spp, from a variety of clinical specimens. Thayer Martin is used for the selective isolation of pathogenic Neisseria from specimens with mixed flora of bacteria and fungi.
Does Neisseria gonorrhoeae grow on EMB agar?
While the plate on the right selectively only allows the bacteria Neisseria gonorrhoeae, to grow (white dots). Eosin methylene blue (EMB) that contains methylene blue – toxic to Gram-positive bacteria, allowing only the growth of Gram negative bacteria.
What is Stuart transport medium used for?
Remel Stuart Transport Medium is a semisolid medium recommended for use in the transport and preservation of microbiological specimens. Stuart Transport Medium is a nonnutritional medium which maintains the viability of organisms without significant multiplication.
Does Neisseria gonorrhoeae grow on starch agar?
Dextrose Starch Agar is used for propagating pure cultures of Neisseria gonorrhoeae. Store prepared media below 8°C, protected from direct light. Store dehydrated powder in a dry place in tightly-sealed containers at 2-25°C.
Does Neisseria gonorrhoeae grow on chocolate agar?
N. gonorrhoeae are gram negative diplococcic that grow on solid media in 24-48 hours when incubated under aerobic conditions at 35-37°C with 5% CO2. They exhibit more robust growth on chocolate agar, but can grow on 5% sheep blood agar as well (Figure 1A and B). Like most other Neisseria spp., N.
What is the selective medium for growing Neisseria gonorrhoeae?
Can Neisseria gonorrhoeae grow on starch agar?
Can Neisseria gonorrhoeae grow on blood agar?
Neisseria gonorrhoeae are the most fastidious of the Neisseria species, require complex growth media and are highly susceptible to toxic substnces (e.g., fatty acids). Gonococci are not able to grow on common blood agar.
Which agar promotes the growth of N. gonorrhoeae and N. meningitidis?
chocolate agar plate
N. meningitidis is a fastidious organism, which grows best at 35-37°C with ~5% CO2 (or in a candle-jar). It can grow on both a blood agar plate (BAP) and a chocolate agar plate (CAP).