Table of Contents
What is the well of the microtiter plate?
A microplate typically has 6, 12, 24, 48, 96, 384 or 1536 sample wells arranged in a 2:3 rectangular matrix. Some microplates have been manufactured with 3456 or 9600 wells, and an “array tape” product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
How much does a well in a 96 well plate hold?
CulturPlate Microplates
| Well format | 24-well | 96-well |
|---|---|---|
| Well volume | 2.39 mL | 400 µL |
| Recommended working volume | 0.5-2.39 mL | 80 µL-350 µL |
| Height (mm) | 18.70 | 14.60 |
| Length (mm) | 127.80 | 127.76 |
How many mL is a 96 well plate?
96-Well Reinforced Deep-Well Titer Plate Deep-well reinforced titer plate with a 96-well format. Each square well has a volume of 1.4 mL and a working volume of 1.0 mL.
How much liquid can a 96 well hold?
96 Well Assay Plates Each well holds from 320 to 360 µl depending on which well geometry is selected.
How deep is a 96 well plate?
96-Well 600µL Polypropylene Deep Well Plates Made of polypropylene, they offer excellent chemical resistance and will withstand temperatures down to -80°C. Features 0.6mL deep well plate ANSI/SLAS 1-2004:…
What is the minimum volume of 96-well plate?
The minimum required volume for 96-well microplates is 100 µL, for a half area 96-well plate is 50 µL and for a 384-well plate is 20-25 µL. Sample sizes below those volumes have to be diluted to at least the required minimum volume with the diluent.
How does a 96 well plate reader work?
A microplate reader detects light signals produced by samples which have been pipetted into a microplate. The optical properties of these samples are the result of a biological, chemical, biochemical or physical reaction. Different analytic reactions result in different optical changes used for analysis.
What is microtiter plate assay?
The microtiter dish assay is an important tool for the study of the early stages in biofilm formation, and has been applied primarily for the study of bacterial biofilms, although this assay has also been used to study fungal biofilm formation.
Can you reuse 96 well plates?
These plates are not meant to be reused. In our experience when you proceed to autoclave, and repeat an experiment, the results are not the same, if we make a triplicate of known samples, you can give only one or two reference values.
Are all 96 well plates the same size?
The length of the 96-well standard microplate is 127.71 mm x the width 85.43 mm and the height is 14.10 mm (shown in fig….ANSI- /SLAS – Standard 1 to 4 – 2004.
| 96-Well Standard Plate Dimensions | |
|---|---|
| W | 85.43 mm |
| H | 14.10 mm |
| X1 | 14.36 mm |
| Xn | 113.36 mm |