Table of Contents
What is lactophenol cotton blue staining?
Lactophenol cotton blue (LCB) is a mixture of methyl blue, a histological stain, and lactophenol (a solution of phenol, lactic acid, and glycerol in water). It is used in wet-mount preparations for visualization of fungal structures, especially in medical mycology.
What is lactophenol cotton blue used for?
Lactophenol cotton blue wet mount preparation is commonly used for microscopic identification of fungi. It is formulated with lactophenol, which serves as the mounting fluid and the dye, cotton blue. Lactic acid preserves the fungal structure and clears the tissue while phenol acts as a disinfectant.
Is lactophenol cotton blue basic stain?
Lactophenol Cotton Blue is used as staining solution for fungi. 1) Place a drop of Lactophenol Cotton Blue reagent on a clean and dry slide. The stain imparts a blue colouration on hyphae. 2) By using a nichrome inoculating wire, carefully tease the fungal culture, into a thin preparation.
Why is Lactophenol used for Molds?
Remel Lactophenol is a mounting fluid recommended for use in qualitative procedures to prepare fungal specimens for microscopic examination. Phenol precipitates cytoplasmic proteins and inactivates enzyme systems within the fungal cell. Glycerol prevents drying and lactic acid is a clearing agent.
Is iodine used to identify fungus?
The history of iodine use for identification of fungi dates back to the mid 1800s; its use for white spore identification was described by Melzer in 1924. The production of the positive amyloid reaction is due to an amylose-iodine complex.
How does Lactophenol aniline blue work?
Remel Lactophenol Aniline Blue is a stain recommended for use in wet mount preparations for microscopic examination of fungi. Phenol precipitates cytoplasmic proteins and inactivates enzyme systems within the fungal cell. Glycerol prevents drying, allowing for examination of the stain 18-24 hours after preparation.
How does lactophenol aniline blue work?
What is the function in glycerol in the composition of Lactophenol cotton blue stain?
method used in the laboratory. Main components of lactophenol cotton blue are phenol, lactic acid,glycerol and cotton blue. Phenol acts as a disinfectant, lactic acid preserves the morphology of fungus, glycerol acts as a hygroscopic agent which prevents drying and cotton blue stains the outer wall of fungus.
Does iodine expire?
Again, though the antiseptic will slowly lose potency, it does not “go bad”. If the color looks noticeably lighter, I’d discard it to get a new bottle. That said, iodine is not a perfect bactericidal or fungicide.
What is Riddell slide technique?
The best method for observing sporulation is the slide culture technique described by Riddell in 1950. 1 Riddell’s method required using an agar block of media transferred to a glass slide and placed in a moist chamber. A modified technique developed by Harris in 1986 utilized a layer of water agar to provide humidity.
Should I put iodine on an open wound?
Do not use topical iodine on deep, puncture wounds, animal bites, or serious burns.
Does iodine burn your skin?
Strong solution of iodine is corrosive and can cause blistering and necrosis of skin, commonly referred to as chemical burns or irritant contact dermatitis.
What is lactophenol cotton blue solution used for?
The lactophenol cotton blue solution acts as a mounting solution as well as a staining agent. The solution is clear and blue in color and it is made up of a combination of three main reagents: Phenol: It acts as a disinfectant by killing any living organisms Lactic acid: To preserve the fungal structures
How do you stain Lactophenol cotton blue?
Procedure of Lactophenol Cotton Blue (LPCB) Staining On a clean microscopic glass slide, add a drop of 70% ethanol Add the fungal specimen to the drop of alcohol using a sterile mounter such as an inoculation loop (from solid medium), depending on the sample of use.
How to identify Scopulariopsis species using lactophenol?
Figure: First: Scopulariopsis species on a lactophenol cotton blue stain. Image Source: stylish_streaking and Senthil Prabhu. It can only be used as a presumptive identification method of fungi which should be followed up with other diagnostic tools such as biochemical and cultural examination.