Table of Contents
What Alexa 633?
Alexa Fluor™ 633 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 633 dye is water soluble and pH-insensitive from pH 4 to pH 10.
Are Alexa Fluor tandem dyes?
Additionally, we have conjugated allophycocyanin to Alexa Fluor 680, Alexa Fluor 700 or Alexa Fluor 750 dyes to create tandem conjugates for multicolor measurements using excitation sources in the 633 to 650 nm range (Figure 1.3. 25).
How long do Alexa Fluor antibodies last?
2 weeks
What temperature should I store the antibody at? Storage at 4°C should not exceed 1 or 2 weeks.
Can I use PE and FITC?
In some experiments FITC may be combined with other dyes, for example PE, that emit yellow and orange photons. In those cases the relative contribution of each fluorophore to the signal in a given detector must be determined (Figure 11).
What is Black Hole Quencher?
Black Hole Quencher™ dyes are true “dark” quenchers with no fluorescent signal, improving signal to noise ratios. Through a combination of FRET and static quenching, they deliver results without the residual background signal common to other quenchers.
How long do secondary antibodies last?
Ready-to-use liquid antibody concentrates need to be stored between 4 and 8°C and are stable for up to a year if stored according to the manufacturer’s instructions.
Can I use PE Cy5 and PE cy7 together?
If you need to use two fluorochromes that are not well going together, please put them on markers expressed by different cell populations! Avoid using tandem dyes (PeCy7, PeCy5.
Is FITC same as 488?
Fluorescent conjugates The Invitrogen Molecular Probes Alexa Fluor 488 dye—with spectral properties and quantum yield nearly identical to those of fluorescein isothiocyanate (FITC)—produces brighter, more photostable conjugates.
What is Alexa Fluor 633 dye?
Alexa Fluor® 633 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor® 633 dye is water soluble and pH-insensitive from pH 4 to pH 10.
What is Alexa Fluor secondary antibody?
Alexa Fluor® Secondary Antibodies. Alexa Fluor® fluorescent dyes are widely recognized as superior fluorescent dyes available for conjugation. They are highly water soluble and remain fluorescent from pH 4 to pH 10.
Is Alexa Fluor visible to the human eye?
Fluorescence of this long-wavelength Alexa Fluor® dye is not visible to the human eye but is readily detected by most imaging systems.
Which anti-mouse IgG IgG products are light chain specific?
Alexa Fluor® 790-goat anti-mouse IgG, Fcγ Subclass 1 specific (min X Hu, Bov, Rb Sr Prot, 115-655-205 ) (green) shows heavy chains and Alexa Fluor® 680-goat anti-mouse IgG, light chain specific (min X Bov, Gt, Hrs, Hu, Rb, Rat, Shp Ig, 115-625-174 ) (red) shows light chains. Imaged using LiCor Odyssey imager.