Table of Contents
What is bismuth sulfite agar used for?
Bismuth sulfite agar is a modification of the original Wilson and Blair selective diagnostic medium used for the isolation of Salmonella typhi and other salmonellae from food and other materials, sewage and water. Bismuth sulfite and brilliant green are the selective agents.
How does bismuth sulfite agar selective for Salmonella and Shigella species?
The presence of bismuth sulfite indicator and brilliant green inhibits the growth of Gram-positive bacteria and members of the coliform group while allowing Salmonella to grow luxuriantly. Beef extract and peptones provide nitrogen, vitamins, and minerals needed for the growth of microorganisms.
How do you make bismuth sulfite agar?
Preparation and Method of Use of Bismuth Sulphite Agar (BSA)
- Suspend 52.33 grams in 1000 ml distilled water.
- Heat to boiling to dissolve the medium completely.
- DO NOT STERILIZE IN AUTOCLAVE or by fractional sterilization since overheating may destroy the selectivity of the medium.
Is bismuth sulfite agar selective?
Our Bismuth Sulfite Agar is a selective and differential medium used for the isolation of Salmonella species from a variety of samples including foods and clinical specimens.
What type of media is bismuth sulfite agar?
Bismuth sulfite agar is a type of agar media used to isolate Salmonella species. It uses glucose as a primary source of carbon. BLBG and bismuth stop gram-positive growth. Bismuth sulfite agar tests the ability to use ferrous sulfate and convert it to hydrogen sulfide.
What is Wilson and Blair Medium?
Selective agar introduced by WILSON-BLAIR (1927) for the isolation and differentiation of Salmonella typhi and other Salmonella species from clinical specimens, food and water. This medium complies with the recommendations of the United States Pharmacopeia.
What type of medium is bismuth sulfite agar?
selective medium
Bismuth Sulphite Agar is a selective medium for the isolation and preliminary identification of Salmonella typhi and other salmonellae from pathological material, sewage, water supplies, food and other products suspected of containing these pathogens.
What is Wilson Blair agar?
Wilson and Blair Agar was formulated by Wilson and Blair (1) for isolating Salmonella species especially Salmonella. serotype Typhi from clinical specimens. Peptone and HM peptone B provide nitrogenous, carbonaceous compounds and other growth nutrients. Brilliant green dye. inhibits all gram-positive bacteria.
What is the purpose of Cary and Blair medium?
Cary-Blair Medium is recommended for the collection and transport of fecal and rectal samples, maintaining viability of Salmonella and Shigella in fecal samples. This medium has a low oxidation/reduction potential, which assures bacterial survival for long periods of time.
What is Cary Blair media?
Cary-Blair Transport Medium is a simple, semi-solid, non-nutritive medium used for the collection and preservation of microbiological specimens. The minimal nutrients in the medium facilitate the survival of organisms without multiplication.
What is Cary-Blair transport media?
What is the purpose of bismuth sulfite agar?
Bismuth sulfite agar is a type of agar media used to isolate Salmonella species. It uses glucose as a primary source of carbon. BLBG and bismuth stop gram-positive growth. Bismuth sulfite agar tests the ability to use ferrous sulfate and convert it to hydrogen sulfide .
What is bismuth sulfide used for?
Bismuth Sulfide is a crystalline solid used as a semiconductor and in photo optic applications.
What is the formula for bismuth (III) sulfide?
Bismuth (III) sulfide can be prepared by reacting a bismuth (III) salt with hydrogen sulfide : 2Bi 3+ +3H 2 S → Bi 2 S 3 + 6H + Bismuth (III) sulfide can also be prepared by the reaction of elemental bismuth and elemental sulfur in an evacuated silica tube at 500 °C for 96 hours. 2Bi + 3S → Bi 2 S 3
What is the electron configuration of bismuth?
See more Bismuth products. Bismuth (atomic symbol: Bi, atomic number: 83) is a Block P, Group 15, Period 6 element with an atomic radius of 208. 98040. The number of electrons in each of Bismuth’s shells is 2, 8, 18, 32, 18, 5 and its electron configuration is 4f 14 5d 10 6s 2 6p 3.