What is Rox and why use it in qPCR?
Since ROX is a passive fluorescent dye, the main reason it is used in qPCR is for signal normalisation. By normalising the fluorescent signal, this can reduce the variability between technical replicates. Slight variations between wells can affect the fluorescent signal generated in qPCR.
What is low Rox?
In “low ROX” instruments, you get optimal excitation of the ROX dye at ~575 nm and hence a strong ROX signal. In instruments with a “high ROX” requirement, sub-optimal excitation of the ROX dye at ~488 nm leads to a weak ROX signal, which can be overcome by using a high concentration of ROX (Figure 2A).
What is the wavelength of Rox?
ROX (carboxy-X-rhodamine) is a fluorescent compound with an excitation peak at 578 nm and an emission peak at 604 nm.
Does Quantstudio need Rox?
yes, Quantstudio 3 works with low ROX reference. We have not tried it without ROX, but we never had any problems like this with low ROX PCR mixes (no “colapse” of curves observed even after 40 cycles).
What is reporter dye in PCR?
In real time PCR, DNA binding dyes are used as fluorescent reporters to monitor the real time PCR reaction. The fluorescence of the reporter dye increases as the product accumulates with each successive cycle of amplification.
What is quencher and reporter?
The “reporter” (R) dye is attached at the 5′-end of the probe sequence while the “quencher” (Q) dye is synthesized on the 3′-end. A popular combination of dyes is FAM or VIC for the reporter dye and TAMRA for the quencher dye.
What is Rox in qPCR?
What is ROX? ROX, also known as carboxyrhodamine, is an inert fluorescent dye that can be added to the qPCR master mix. Unlike reporter dyes in qPCR (e.g. SYBR Green), ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions.
Why is Rox called a passive dye in qPCR?
Unlike reporter dyes in qPCR (e.g. SYBR Green), ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions. In other words, the fluorescent signal of ROX remains stable throughout the qPCR process and it is not related to the amount of template in the reaction.
Does ROX-Filer have a copy queue feature?
Rox-Filer is a fine piece of software, but i miss a copy queue feature. i think many other users would appreciate it. Loginto post comments The reasons why Rox filer “cant” replace Nautilus for me
What do you like most about ROX Filer?
Rox-Filer is a fine piece of software, but i miss a copy queue feature. i think many other users would appreciate it. Loginto post comments The reasons why Rox filer “cant” replace Nautilus for me Submitted by Anonymous on Sat, 2008-12-20 04:59. In general I really like Rox filer.