Table of Contents
What is tyrosine recombinase?
Tyrosine site-specific recombinases (T-SSRs) are DNA modifying enzymes that bind, cleave, strand exchange, and rejoin DNA at their respective, typically palindromic, recognition target sites.
What is serine recombinase?
Serine recombinases are often tightly controlled by elaborate, topologically-defined, nucleoprotein complexes. Hin is a member of the DNA invertase subclass of serine recombinases that are regulated by a remote recombinational enhancer element containing two binding sites for the protein Fis.
What is attP sites in Drosophila?
The attP site (5′-GTGCCCCAACTGGGGTAACCTttGAGTTCTCTCAGTTGGGGG-3′) is one of the two target sites of the phiC31:int integrase. phiC31:int mediates recombination between two non-identical target sites, attP and attB, each of which contains a short integration core flanked by imperfect inverted repeats.
What is attL and attR?
The attL and attR sites are substrates for the excision reaction, and each is composed of one B-type half-site and one P-type half-site.
How do you get Cre infection?
CRE are usually spread person to person through contact with infected or colonized people, particularly contact with wounds or stool (poop). This contact can occur via the hands of healthcare workers, or through medical equipment and devices that have not been correctly cleaned.
What is tyrosine integrase?
The integrases are a diverse family of tyrosine recombinases which rearrange DNA duplexes by means of conservative site-specific recombination reactions.
Which of the following is an example of serine recombinase?
Famous serine recombinases include enzymes such as gamma-delta resolvase (from the Tn1000 transposon), Tn3 resolvase (from the Tn3 transposon), and φC31 integrase (from the φC31 phage).
What are serine integrases?
Serine integrases catalyze the integration and excision of phage genomes into and out of bacterial chromosomes in a highly specific and directional manner, making these proteins powerful tools for genome engineering. In 2013, the first structure of a serine integrase-DNA complex was reported.
What are attP sites?
What is LoxP site?
LoxP sites are sites recognized by the bacteriophage cre recombinase, which excises sequences lying between two loxP sites leaving behind only one of the two sites.
What is an ATT site?
The minimal phage att site is composed of approximately 240-base pairs and four distinct binding sites for Int protein, at least three of which are crucial for function. This ‘donor site’ recombines efficiently with a smaller ‘recipient site’ that lacks the extensive interactions with Int protein.
What is BP and LR reaction?
The BP reaction creates an attL-flanked entry clone. The LR reaction creates an expression clone with all of the components necessary for gene expression. The LR Reaction takes place between the attL sites of the generated entry clone and the attR sites of the destination vector.
What is the minimum size of attB and attP?
This assay system was also used to define the minimal sizes of attB and attP at 34 bp and 39 bp, respectively. Furthermore, precise and efficient intermolecular integration of an incoming plasmid bearing attP into an established Epstein-Barr virus plasmid bearing attB was documented in human cells.
Which recombination directionality Factor (RDF) is required for reverse Attl X ATTR excisive reactions?
The reverse attL x attR excisive reaction also requires Int as well as a recombination directionality factor (RDF), named Xis in the HK system and gp3 in the ΦC31 system [ 6 ], typically supplied in trans from a helper plasmid, a non-replicating DNA molecule, or as mRNA [ 7 ].
Can BLA be used as a reporter for recombination?
The HK/ΦC31 att – bla system offers a simple way to directly select recombination events, thus enhancing the use of site-specific recombination systems for carrying out precise, large-scale DNA manipulation, and adding useful tools to the genetics toolbox. We further show the power and flexibility of bla to be used as a reporter for recombination.
Does amino terminus modification affect protein export in Escherichia coli?
Li P, Beckwith J, Inouye H. Alteration of the amino terminus of the mature sequence of a periplasmic protein can severely affect protein export in Escherichia coli. Proc Natl Acad Sci U S A. 1988;85 (20):7685–9.