What does N6-Methyladenosine do?
As one of the most abundant mRNA modifications, N6-methyladenosine (m6A) is involved in the regulation of multiple aspects of RNA biology, including RNA decay, splicing, and translation.
What is m6A RNA?
N6-methyladenosine, otherwise known as m6A, is the most abundant internal modification in mRNA. It entails methylation of the adenosine base at the nitrogen-6 position. Previous studies have demonstrated that m6A is a reversible and dynamic RNA modification that is integral to the regulation of RNA metabolism.
Where is m6A?
m6A RNA methylation regulates CNS development Emerging studies have indicated that m6A is more abundant in the CNS than that in other organs, which increases in overall abundance from the embryonic to adult brain, suggesting its critical roles during normal brain development and function40.
What does RNA methylation do?
This study confirmed the important role of RNA methylation in promoting UV resistance of cells, and found a new pathway in which METTL3, m6A RNA and “Pol k” play an important role in the early stage of UV induced DDR response, and RNA methylation is crucial for the recruitment of “Pol k” to the damage site [51].
What is the N6 position?
Abstract. N6-methyladenosine (m6A) is methylation that occurs in the N6-position of adenosine, which is the most prevalent internal modification on eukaryotic mRNA.
What is m6A RNA methylation?
m6A methylation regulates gene expression by influencing numerous aspects of mRNA metabolism, including pre-mRNA processing, nuclear export, decay, and translation.
Who discovered m6A?
Erasers. FTO is the first discovered demethylase that removes methyl groups from m6A (19), indicating that m6A is a dynamically reversible RNA modification.
When was m6A discovered?
1970s
N6-Methyladenosine (m6A) was originally identified and partially characterised in the 1970s, and is an abundant modification in mRNA and DNA. It is found within some viruses, and most eukaryotes including mammals, insects, plants and yeast.
What do m6A readers do?
Apart from being involved in RNA metabolism, m6A readers also participate in different biological processes, including tumorigenesis, hematopoiesis, virus replication, immune response, and adipogenesis18.
Where does m6A modification occur?
Regulation of m6A modification m6A modification mainly occurs in adenines in the RRACH sequence, and its functions are implemented by RNA methyltransferases (writers), RNA demethylase (erasers) and m6A binding protein (readers).
Is m6A epigenetic?
N6-methyladenosine (m6A) is an epigenetic modification of RNA molecules that was discovered in the early 1970s in messenger RNAs (mRNAs) from eukaryotes.
What is the role of N6-methyladenosine in the stability of RNA-DNA hybrids?
“N 6 -methyladenosine regulates the stability of RNA:DNA hybrids in human cells”. Nature Genetics. 52 (1): 48–55. doi: 10.1038/s41588-019-0549-x.
How many types of m6A methylation are there?
“Perturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5′ sites”. Cell Reports. 8 (1): 284–96. doi: 10.1016/j.celrep.2014.05.048.
Is N6-methyladenosine an epigenetic player in determination of stem cell fate?
“N6-Methyladenosine in RNA and DNA: An Epitranscriptomic and Epigenetic Player Implicated in Determination of Stem Cell Fate”. Stem Cells International. 2018: 3256524. doi: 10.1155/2018/3256524.
What do m6A mRNA methylomes tell us about epitranscriptomic control of cardiac tissue?
First m6A mRNA methylomes of human cardiac tissue from healthy patients and heart failure patients and of animal model heart samples were reported and shed point toward an important role of epitranscriptomic control on cell growth.