Table of Contents
What is the solid phase in ion exchange chromatography?
In cation-exchange chromatography, the stationary phase, which consists of a large quantity of acid groups attached to a polymeric resin, is slurried with water and applied to a column. The mobile phase, which contains the inorganic salt dissolved in a suitable solvent, is applied to the column.
What is solid phase extraction method?
Solid phase extraction (SPE) is a technique designed for rapid, selective sample preparation and purification prior to the chromatographic analysis (e.g. HPLC, GC, TLC). In SPE, one or more analytes from a liquid sample are isolated by extracting, partitioning, and/or adsorbing onto a solid stationary phase.
Why do we use solid phase extraction?
Solid phase extraction prevents most problems encountered with liquid-liquid extraction and improves quantitative recovery yields. This technique is rapid (most extraction in less than 30 min), easy to perform and can be automated.
What are the benefits of SPE?
As an SPE member, you’ll receive:
- conference discounts;
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- a wealth of resources for networking, knowledge building, career advancement, and streaming content;
What are the disadvantages of SPE?
Three of the most common problems seen with solid-phase extraction (SPE) are poor recovery, reproducibility issues, and sample extracts being insufficiently clean.
Which is ion exchange phase SPE media?
Ion-exchange media come in both anionic and cationic forms for the extraction of analytes with basic or acidic functional groups. Cation-exchange sorbents contain surface groups that are negatively charged, and the reverse is true for anion-exchange materials.
What is the purpose of ion exchange chromatography?
Ion exchange chromatography is commonly used to separate charged biological molecules such as proteins, peptides, amino acids, or nucleotides. The amino acids that make up proteins are zwitterionic compounds that contain both positively and negatively charged chemical groups.
What is SPE sample preparation?
What is SPE? Solid phase extraction (SPE) is a sample preparation technique often used by chromatographers prior to analysis. SPE is most often used to remove interfering compounds from a sample, although it can also be used to enrich/concentrate analytes of interest in the sample.
What is SPE separation?
Solid-phase extraction (SPE) is an extractive technique by which compounds that are dissolved or suspended in a liquid mixture are separated from other compounds in the mixture according to their physical and chemical properties.
What are SPE cartridges?
Solid-Phase Extraction (SPE) is a sample preparation technique that is used to clean up and/or concentrate samples before analysis. Compared to liquid-liquid extraction, SPE is much faster, uses less solvent, and saves money. SPE provides clean extracts and high recoveries.
Is HPLC solid-phase extraction?
Good sensitivity and short retention times for drugs were obtained with the HPLC method using the solid-phase extraction procedure proposed.
What is solid phase extraction in microbiology?
Solid Phase Extraction (SPE) Solid phase extraction1 (SPE) is a sample preparation technique using a solid adsorbent contained most commonly in a cartridge device (Figure 1), or on a disk to adsorb select species from solution. SPE is used to isolate a species in a sample or to clean-up a sample before analysis. Figure 1.
What is the ion exchange media used in SPE column?
Table 1. Ion exchange media to use for different analyte types. Because the sorbents in the strong ion exchange SPE columns have strong acid or base groups, these sorbents are 100% ionized at all pH’s. Therefore, to control the charge, the pH of the analyte needs to be adjusted to turn it on or off.
What is the combination of reversed phase and ion-exchange?
Combination of reversed-phase and ion-exchange for mixed-mode Table 1. Ion-Exchange & Mixed-Mode SPE Counter ion selectivity is defined as the degree to which a counter ion is capable of competing with other counter ions for the functional group of an ion exchanger sorbent.
What is ionized ion exchange for electrostatic retention?
In order for electrostatic retention to occur, both analyte and sorbent functional groups must be in their ionizedIon Exchange Methodology form. This is done through strict pH control of the sample matrix.