Table of Contents
How would you prepare a 1/10 dilution of a serum sample?
For example, to make a 1:10 dilution of a 1M NaCl solution, you would mix one “part” of the 1M solution with nine “parts” of solvent (probably water), for a total of ten “parts.” Therefore, 1:10 dilution means 1 part + 9 parts of water (or other diluent).
What would you do to a bacterial culture to get a 1/10 dilution plate?
How would you inoculate a plate to get a 1:10 dilution? -Plate 0.1 ml of culture. -Mix 1 ml of a culture with 9 ml of water and plate 1 ml of the dilution. You just studied 8 terms!
What is the procedure for serial dilution?
Procedure of Serial Dilution 1 ml of properly mixed sample/culture is drawn into the pipette. The sample is then added to the first tube to make the total volume of 10 ml. This provides an initial dilution of 10-1. The dilution is thoroughly mixed by emptying and filling the pipette several times.
How is serial dilution performed?
Serial Dilution Formula/Calculations Serial dilution involves the process of taking a sample and diluting it through a series of standard volumes of sterile diluent, which can either be distilled water or 0.9 % saline. Then, a small measured volume of each dilution is used to make a series of pour or spread plates.
How much saline do I need for a 1/10 dilution?
Simple Dilution 1: 10 dilution = ratio of 1 : 9 = 1 : 10 = one part of serum + 9 parts of diluent. 100 μL + 900 μL of saline. 1 mL serum + 9 mL of saline. 2 mL serum + 18 mL of saline.
Why is serial dilution usually performed with a power of 10 dilutions?
Objectives of Serial Dilution Serial dilutions are commonly performed to avoid having to pipette very small volumes (1-10 µl) to make a dilution of a solution.
What would you do to a bacterial culture to get a 1/10 dilution plate 1/100 dilution quizlet?
How would you inoculate a plate to get a 1:10 dilution? AND a 1:100 dilution? Just plate 0.1ml for a 1:10. To do a 1:100 do a 1:10 dilution then plate 0.1 dilution then plate 0.1 ml.
How do you perform a serial dilution?
To perform a serial dilution, a small amount of a well-mixed solution is transferred into a new container, and additional water or other solvent * is added to dilute the original solution. The diluted sample is then used as the base solution to make an additional dilution.
How do you do a 1/20 dilution?
For example, a 1:20 dilution converts to a 1/20 dilution factor. Multiply the final desired volume by the dilution factor to determine the needed volume of the stock solution. In our example, 30 mL x 1 ÷ 20 = 1.5 mL of stock solution.
How do you do a 1/5 serial dilution?
Answer: 1:5 dilution = 1/5 dilution = 1 part sample and 4 parts diluent in a total of 5 parts. If you need 10 ml, final volume, then you need 1/5 of 10 ml = 2 ml sample. To bring this 2 ml sample up to a total volume of 10 ml, you must add 10 ml – 2 ml = 8 ml diluent. 2.
How would you prepare 5 ml of a 1/10 dilution of serum?
A series of five dilutions is made of this first dilution by diluting it 1/10, re-diluting 1/10, and then three times more, each resulting solution then being a 1/10 dilution of the previous one in the series. The concentration of serum in each solution is as follows: 1/2, 1/20, 1/200, 1/2,000, 1/20,000, and 1/200,000.
How much diluent should be used in order to create a 1/10 dilution using a sample volume of 5 ml?
How much initial sample and diluent should you use? Answer: 1:5 dilution = 1/5 dilution = 1 part sample and 4 parts diluent in a total of 5 parts. If you need 10 ml, final volume, then you need 1/5 of 10 ml = 2 ml sample. To bring this 2 ml sample up to a total volume of 10 ml, you must add 10 ml – 2 ml = 8 ml diluent.
What is a 10 times dilution?
A ten-fold dilution reduces the concentration of a solution or a suspension of virus by a factor of ten that is to one-tenth the original concentration. A series of ten-fold dilutions is described as ten-fold serial dilutions.
How would you inoculate a plate to get a 1/10 dilution?
When 1.0 ml of a sample is added to 9.0 ml of sterile media what is the dilution?
1. Add 1.0ml of the original broth culture to 9.0ml water (to obtain a 10-1dilution). The 10-1 dilution will have 600 cells/ml.