Table of Contents
What does IL 6 do?
Interleukin 6 (IL-6), promptly and transiently produced in response to infections and tissue injuries, contributes to host defense through the stimulation of acute phase responses, hematopoiesis, and immune reactions.
How do you detect il6?
Currently, ELISA and western blot is the staple detection technique for IL-6. However, they require substantial time, cost, machinery and specialist training. On the other hand, a Graphene Oxide-based amperometric sensor can provide real-time, low-cost yet sensitive protein detection.
Does IL-6 cause inflammation?
IL-6 is involved both in immune responses and in inflammation, hematopoiesis, bone metabolism and embryonic development. IL-6 plays roles in chronic inflammation (closely related to chronic inflammatory diseases, autoimmune diseases and cancer) and even in the cytokine storm of corona virus disease 2019 (COVID-19).
What is the role of IL-6 in COVID-19 disease progression?
Our results demonstrate that increasing the levels of IL-6 correlate to disease severity and identifying particularly well those patients who evolved to more severe stages of COVID-19, a pattern not observed with other markers such as CRP.
What does raised IL-6 mean?
Therefore, IL-6 serum levels greater than 35 pg/mL of IL-6 are associated with increased risk of mortality, mechanical ventilation requirements, and increased severity of SARS-CoV-2 induced pneumonia.
What is the opteia set for mouse interleukin-6 (IL-6)?
The OptEIA™ Set for mouse interleukin-6 (IL-6) contains the components necessary to develop enzyme-linked immunosorbent assays (ELISA) for natural or recombinant mouse IL-6 in serum, plasma, and cell culture supernatants.
What cells secrete IL-6?
Created with sketchtool. Description: Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a ~21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6.
What is the intended use of the mouse IL-6 lyophilized standard?
Applications Tested: The intended use of the Mouse IL-6 Lyophilized Standard is as a standard for Mouse IL-6 ELISA (cat. 88-7064). Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard.
How do you find the IL-6 concentration of unknowns?
To determine the IL-6 concentration of the unknowns, find the unknown’s mean absorbance value on the y-axis and draw a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the x-axis and read the IL-6 concentration.