Table of Contents
What is 1kb DNA Ladder?
1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 – 10,000 base pairs (bp). The ladder consists of 13 double-stranded, blunt-end fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1).
What is the DNA Ladder 1 KB 1.5kb ladder?
Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.
Is bp the same as kb?
bp = base pair(s) kb (= kbp) = kilo base pairs = 1,000 bp. Mb = mega base pairs = 1,000,000 bp. Gb = giga base pairs = 1,000,000,000 bp.
What is a 100 bp ladder?
Invitrogen 100 bp DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 2,000 bp. 100 bp DNA Ladder consists of 13 individual chromatography-purified DNA fragments and has reference bands at 2000, 1500, and 600 bp for easy orientation.
How much DNA do you need to see on gel?
How much DNA should be loaded per well of an agarose gel? The amount of DNA to load per well is variable. The least amount of DNA that can be detected with ethidum bromide is 10 ng. DNA amounts of up to 100 ng per well will result in a sharp, clean band on an ethidium bromide stained gel.
What is DNA size marker?
Essentially, a typical DNA size marker comprises from discrete DNA fragments of specific size ranging from tens to thousands of base pairs (bp) where application of these to agarose gel provides a visual reference to help to estimate size of unknown DNA molecules (2).
What is the 50 bp DNA ladder used for?
Thermo Scientific GeneRuler 50 bp DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 50 bp to 1,000 bp on agarose or polyacrylamide gels. The DNA ladder consists of 13 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.
What is Invitrogen ladder 50 bp?
Product overview. Description. Invitrogen 50 bp DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 50 bp to 2,500 bp. 50 bp DNA Ladder consists of 17 individual chromatography-purified DNA fragments and has reference bands at 2500, 800, and 350 bp for easy orientation.
How do I prepare 50 bp DNA step ladder for Na electrophoresis?
Sigma′s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
What is generuler 1 KB plus DNA ladder?
Description GeneRuler 1 kb Plus DNA Ladder 75 to 20,000 bp is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose gels. The ladder is a mixture of chromatography-purified individual DNA fragments. GeneRuler 1 kb Plus DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase.