Table of Contents
What is beta-catenin positive?
In summary, high-level nuclear β-catenin staining may be useful as a diagnostic marker for desmoid-type fibromatosis, as only a very limited subset (solitary fibrous tumor, synovial sarcoma, and endometrial stromal sarcoma) of other mesenchymal neoplasms are positive for this marker.
What does B catenin do?
In the canonical Wnt cascade, β-catenin is the key effector responsible for transduction of the signal to the nucleus and it triggers transcription of Wnt-specific genes responsible for the control of cell fate decisions in many cells and tissues.
Is beta-catenin good?
β-catenin is an intracellular molecule involved in cell adhesion, cell signaling, and regulation of gene transcription. It plays essential roles in kidney development and in the pathogenesis of renal dysplasia.
What is active B catenin?
Non-phospho (Active) β-Catenin (Ser33/37/Thr41) Antibody recognizes endogenous β-Catenin when residues Ser33, Ser37, and Thr41 are not phosphorylated. It does not detect β-Catenin if tri-phosphorylated at Ser33/Ser37/Thr41. This antibody may also detect β-Catenin protein when singly phosphorylated at Ser33.
How is B catenin regulated?
β-Catenin is a pivotal component of the Wnt signaling pathway and it is tightly regulated at three hierarchical levels: protein stability, subcellular localization and transcriptional activity.
Is β-catenin an oncogene?
As a key WNT pathway oncogene41, β-catenin also controls transcription of multiple target genes42 via its binding to transcription factors including lymphoid enhancement factor (LEF-1) and T-cell factor (TCF4).
How is beta-catenin degraded?
Ubiquitinated β-catenin is degraded by the proteasome. The molecular mechanisms that underlie several aspects of destruction complex function are poorly understood, particularly the role of APC.
What is Wnt β catenin signaling?
Wnt/β-catenin signaling, a highly conserved pathway through evolution, regulates key cellular functions including proliferation, differentiation, migration, genetic stability, apoptosis, and stem cell renewal.
Does beta catenin have a NLS?
β-Catenin contains no recognizable NLS and NES. The nuclear localization of β-catenin can be enhanced when coexpressed with the lymphoid enhancer factor/T cell factor family of transcription factors, so it has been proposed that β-catenin is imported into the nucleus by a piggyback mechanism (34–36).
What causes the beta catenin?
β-catenin/Wnt Typically, mutations interfere with its phosphorylation by GSK3β and thereby prevent its degradation. In addition, mutations and epigenetic modifications in other proteins of the Wnt/β-catenin signaling pathway, such as in APC and AXIN1, also result in increased β-catenin activity in HCC.
Is Y654 phosphorylated of β-catenin related to transcriptional activity?
Promoter-reporter assays demonstrated that Y654 phosphorylation of β-catenin was closely related to TCF transcriptional activity. In vitro kinase assays, using recombinant FLT3 and biotinylated β-catenin peptide including Y654 showed that FLT3 directly phosphorylated Y654 of β-catenin.
What does Y654 stand for?
Synthetic peptide corresponding to Human beta Catenin (phospho Y654). (A-T-YP-A-A) The Life Science industry has been in the grips of a reproducibility crisis for a number of years.
What is the role of β catenin in Wnt?
β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-Catenin at Ser45.
How does Madin-Darby bovine kidney (MdbK) react with alpha catenin peptide?
In immunoblots, reacts with a 94kD protein in extracts of Madin-Darby Bovine Kidney (MDBK) cultured cells. Specific staining is inhibited following pre-incubation of the antiserum with the beta-catenin peptide. Shows no reactivity with BSA conjugated alpha catenin peptide (amino acids 890-901).