Table of Contents
Is maltase an alpha-glucosidase?
α-Glucosidases (EC 3.2. 1.20) catalyze the hydrolysis of terminal, nonreducing α-1,4-linked glucose residues from aryl (or alkyl)-glucosides, disaccharides, or oligosaccharides. α-glucosidases are frequently named maltases, although some of them may have weak activity on maltose.
What is alpha-glucosidase test?
This test provides diagnostic testing for individuals with decreased alpha-glucosidase activity on newborn screen or clinical signs and symptoms suspicious for Pompe disease.
Can maltose be digested by glucosidase?
Membrane Digestion Maltase-glucoamylase hydrolyzes maltose and malto-oligosaccharides to generate free glucose.
What are the alpha-glucosidase enzymes?
Glucosidase enzymes catalyze hydrolysis of starch to simple sugars. In humans, these enzymes aid digestion of dietary carbohydrates and starches to produce glucose for intestinal absorption, which in turn, leads to increase in blood glucose levels.
What does maltose break down into?
In organisms, maltose is decomposed into two glucose molecules when exposed to the enzyme maltase (α-glucosidase) present in the digestive juices of animals and humans.
What is alpha-glucosidase activity?
Alpha-glucosidases are enzymes in the digestive tract that hydrolyze carbohydrates into glucose. One strategy that has been developed to treat type-2 diabetes is inhibition of the activity of alpha-glucosidases using synthetic drugs. However, these inhibitors are usually associated with gastrointestinal side effects.
What enzyme digests maltose?
Where are alpha-glucosidase found?
small intestine
1.20, maltase, glucoinvertase, glucosidosucrase, maltase-glucoamylase, alpha-glucopyranosidase, glucosidoinvertase, alpha-D-glucosidase, alpha-glucoside hydrolase, alpha-1,4-glucosidase, alpha-D-glucoside glucohydrolase) is a glucosidase located in the brush border of the small intestine that acts upon α(1→4) bonds.
Does maltose react with Benedict’s reagent?
Because the aglycone is a hemiacetal, maltose undergoes mutarotation. For the same reason maltose is a reducing sugar. The free aldehyde formed by ring opening can react with Benedict’s solution.
Is maltose made of alpha or beta glucose?
Maltose which is also known as malt is a disaccharide made up of two alpha D glucose unit. The two-unit of glucose are linked with an alpha 1,4 glycosidic bond.
Is maltose made of alpha glucose?
Maltose is a disaccharide made up of two alpha D- glucose in which C1 of the first glucose unit is bonded to C4 of the second glucose unit. The bond that joined two alpha glucose units is called 1, 4 glycosidic linkages.
What is alpha-glucosidase inhibitory assay?
Is amylase an alpha-glucosidase?
α-amylase begins the process of carbohydrate digestion by hydrolysis of 1, 4-glycosidic linkages of polysaccharides (starch, glycogen) to disaccharides and α-glucosidase catalyzes the disaccharides to monosaccharides, which leads to postprandial hyperglycemia.
Does maltose give a positive Benedict test?
Likewise, some disaccharides such as maltose and lactose contain a hemiacetal. They are also reducing sugars that give a positive Fehlings, Benedict, or Tollens test (picture of lactose positive test is further below).
What is acid maltase deficiency?
Acid maltase deficiency is a metabolic muscle disorder, a group of diseases that interferes with the processing of food (in this case, carbohydrates) for energy production. What are the symptoms of acid maltase deficiency?
Does α-glucosidase activity in blood spots vary by enzyme activity?
In comparison, acid α-glucosidase activity yielded 100% sensitivity and 100% specificity. Of the 17 patient samples used in this study, 16 had no detectable enzyme activity in blood spots, with 1 sample having 0.04 μmol · h −1 · L −1, substantially less than the lower end of the control range (0.3 μmol · h −1 · L −1 ).
Does maltase as a hydrolytic enzyme affect bulb fructan content variation?
Sucrose measured in microplates employing maltase as a hydrolytic enzyme was in agreement with HPLC-PAD results. The method revealed significant variation in bulb fructan content within open-pollinated ‘Pukekohe Longkeeper’ breeding populations over a threefold range.
How accurate is the α-glucosidase assay for the identification of Pompe disease?
The assay showed a sensitivity and specificity of 100% for the identification of Pompe-affected individuals. Conclusions: The determination of acid α-glucosidase activity in dried-blood spots is a useful, noninvasive diagnostic assay for the identification of Pompe disease.