Table of Contents
What are electroporation cuvettes?
Ideal for microbiological studies, the electroporation cuvettes transform bacteria, yeast, mammalian, and plant cell samples by applying an electric field and introducing genes. Featuring the necessary aluminum electrode plates on the sides, the tissue culture filled containers prevent static.
Can you reuse electroporation cuvettes?
The cuvettes should be washed for reuse. If washed properly, they can be reused up to 10 times. For each cuvette, do the following while waiting for the cells to recover: Squirt 95% ethanol to fill cuvette more than halfway.
How do you use electroporation machine?
How electroporation works
- Step 1 : Prepare cells. Prepare cells by suspending in electroporation buffer.
- Step 2 : Apply electrical pulse. Apply electrical pulse to cells in the presence of specialized buffer and nucleic acids.
- Step 3 : Return cells to growing conditions.
- Step 4 : Assay cells.
What is time constant electroporation?
The pulse length in an exponen- tial decay wave system is called the “time constant” which is character- ized by the rate at which the pulsed energy (e) or voltage is decayed to 1/3 the original set voltage. This time constant is modified by adjusting the resistance and capacitance (RC) values in an exponential decay.
What affects electroporation?
Electroporation Conditions Factors that affect efficient electroporation of cells are waveform, pulse duration, field strength, cells and cell density, nucleic acid concentration and type, and electroporation buffer. These conditions can be tested and optimized rapidly using the Gene Pulser MXcell system.
Is electroporation better than heat shock?
Compatible cell types On the other hand, electroporation tends to be more efficient than heat shock. Hence, this method is amenable to a broader range of DNA amounts (from low to saturating concentrations), fragment sizes, and complexities.
How is electroporation performed?
Electroporation is based on a simple process. Host cells and selected molecules are suspended in a conductive solution, and an electrical circuit is closed around the mixture. An electrical pulse at an optimized voltage and only lasting a few microseconds to a millisecond is discharged through the cell suspension.
What are the advantages of electroporation?
The main advantage of electroporation is its applicability for transient and stable transfection of all cell types. Furthermore, because electroporation is easy and rapid, it is able to transfect a large number of cells in a short time once optimum electroporation conditions are determined.
Is electroporation same as heat shock?
Heat Shock Transformation. The advantages of using electroporation are the higher efficiency, more colonies, and much faster transformations compared to heat shock method.
What are electroporation cuvettes used for?
Ideal for microbiological studies, the electroporation cuvettes transform bacteria, yeast, mammalian, and plant cell samples by applying an electric field and introducing genes. Featuring the necessary aluminum electrode plates on the sides, the tissue culture filled containers prevent static.
What is a Zeus cuvettes used for?
Description: Zeus™ electroporation cuvettes provide consistent electroporation results. Description: Designed for use in the electroporation of bacteria, yeast, mammalian, and plant cells. Description: Use for electroporation of bacteria, yeast, or mammalian cells.
Why choose Bulldog Bio’s electroporation cuvettes?
In order to achieve the proper electrical field strength, Bulldog Bio’s electroporation cuvettes are carefully constructed with burr-free, specially-processed electrodes and virgin medical-grade polycarbonate. This results in cuvettes which provide superior results in the most discriminating applications.
How to choose the right Cuvette size for your cell?
Chose from three gap widths—1 mm, 2 mm, and 4 mm (0.1 cm, 0.2 cm, and 0.4 cm)—it’s as simple as choosing the correct cuvette size to suit your cells! We are the guaranteed price leader for electroporation cuvettes. Our prices are lower than all of the companies listed here.