Table of Contents
Can you fix annexin V staining?
In my experience, it is possible to fix annexin V stained cells and see great results on the flow. Stain cells with annexin V (in calcium-rich binding buffer). Wash cells in binding buffer to remove unbound AxV. Fix in your choice of fixative (diluted in binding buffer).
What is the color of apoptotic cells upon staining with annexin V FITC?
When cells are stained with Annexin V and propidium iodide, apoptotic cells expressing PS show green fluorescence, which can be detected in the FITC channel, and low red fluorescence. Dead or necrotic cells show bright red fluorescence and no green fluorescence, while live cells show no green or red fluorescence.
What is annexin V APC?
APC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10e2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids.
What is Annexin FITC?
Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine(PS) during an early apoptosis event where the PS becomes exposed at the cell surface. The TACS Annexin V-FITC Apoptosis Detection Kit is supplied with an optimized Binding Buffer and propidium iodide.
What wavelength is FITC?
FITC emits fluorescence from 475 to 650 nm, peaking at 525 nm, which falls in the green spectrum.
What color is APC?
Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields.
Does FITC overlap APC?
1)Move CD8 to a fluorochrome that has less spectral overlap with PE (such as PerCP-Cy5. 5 or APC). 2)Move CD62L to a detector that is still relatively bright, but does not overlap with FITC (such as APC or PE-Cy5).
What is APC in flow cytometry?
APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability.
What does APC stain for?
General notes. APC is well suited for immunohistochemical and immunofluorescence studies of oligodendrocytes and optic nerves due to the antibody’s staining of the cell body as opposed to the myelinated processes.
Is APC red?
What is 7-AAD viability staining solution used for?
7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion. View more applications data for this product in our Scientific Poster Library. Paterson AM, et al. 2011. J.
What is annexin V-cf blue 7-AAD apoptosis?
Find out more. Annexin V-CF Blue 7-AAD Apoptosis Staining / Detection Kit (ab214663) contains Annexin V labeled with CF Blue, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry.
Why does 7-AAD stain positive on DNA?
Early apoptotic cells will exclude 7-AAD, while late stage apoptotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA. 7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells.
What is 7 AAD used for in flow cytometry?
Description. 7-AAD (7-amino-actinomycin D) is a ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. 7-AAD can be used in place of PI (propidium iodide). This solution can be used in combination with PE (phycoerythrin) and FITC (fluorescein isothiocyanate) conjugated antibodies in 2-color analysis.