How do you tell the difference between HL and 60 cells?
HL-60 cells were differentiated with all-trans retinoic acid (ATRA), dimethyl sulfoxide (DMSO) or dimethylformamide (DMF) and stimulated with phorbol 12-myristate 13-acetate (PMA) or calcium ionophore A23187 (CI). Cell differentiation, phagocytosis and calcium influx were analyzed by flow cytometry.
What are HL-60 cells used for?
HL-60 cells are promyeoloblasts isolated from the peripheral blood by leukopheresis from a 36-year-old, Caucasian female with acute promyelocytic leukemia. This cell line can be used in immune disorder and immunology research. Discounts are available for our fellow nonprofit organizations.
What are HL-60 cells and why are they interesting in the study of apoptosis?
HL-60 cell model was used to study the effect of DNA topoisomerase (topo) IIα and IIβ on differentiation and apoptosis of cells and is especially useful in dielectrophoresis studies, which require an aqueous environment with suspended and round cells.
Where did HL 60 cells come from?
Cell line history HL60 is a human cell line derived from peripheral blood lymphocytes of a 36 year old woman suffering from acute promyleocytic leukaemia. It was one of the first human myeloid leukaemia cells to be established as a continuous suspension cell culture which had previously been difficult to achieve.
What are SMMC 7721 cells?
The SMMC-7721 cell line was derived from an Asian male patient with hepatocellular carcinoma in 1977. Human hepatoma cell line SMMC-7721 expresses EGFR and VEGFR and is an invaluable tool in liver cancer research.
Can APL leukemia be cured?
Because of advances in diagnostic techniques and modern treatments, APL is today considered to be the most curable subtype of acute myeloid leukemia in adults, with complete remission rates of 90 percent and cure rates of approximately 80 percent and even higher among low-risk patients.
How long can you live with APL?
APL is now considered a highly curable disease, with 2-year event-free survival rates of 75–84%. Early mortality is common in APL and is frequently related to hemorrhagic complications. Prior to ATRA therapy, early death (ED) related to hemorrhage occurred in up to 26% of cases.
How long can you live with APL leukemia?
Does APL relapse?
Although APL is remarkably responsive to current therapeutic protocols, with a complete response rate of 90%, up to 30% of patients have a relapse.
How does retinoic acid-treated HL-60 promote apoptosis?
We have found that retinoic acid-treatment of HL-60 cells over a period of 6-8 days resulted in a progressive increase in the proportion of cells with mature neutrophil morphologies and was closely followed by an increase in the proportion of cells exhibiting the morphological characteristics of apoptosis, the non-pathological mode of cell death.
How do you differentiate hlhl-60 cells?
HL-60 cells were differentiated with all-trans retinoic acid (ATRA), dimethyl sulfoxide (DMSO) or dimethylformamide (DMF) and stimulated with phorbol 12-myristate 13-acetate (PMA) or calcium ionophore A23187 (CI). Cell differentiation, phagocytosis and calcium influx were analyzed by flow cytometry.
Are hl-r5 cells resistant to differentiation by retinoic acid?
In HL-R5 cells, resistant to the induction of differentiation by retinoic acid but not DMSO, the characteristic c-myc down-regulation which is associ … The expression of c-myc and two calcium-binding proteins, MRP8 and MRP14, has been analyzed in wild-type and differentiation-resistant HL-60 variants.
Do retinoic acid and DMSO act differently on hl-d4 cells?
The resistance of HL-D4 cells to DMSO and retinoic acid, and the different effects of these agents on c-myc RNA levels, despite their common effect on the expression of MRP8 and MRP14, suggest that the two agents act through different pathways which coverage before the onset of myeloid differentiation in HL-60 cells. Publication types