Table of Contents
How do you treat a Poly-L-Lysine slide?
Coating Procedure:
- Allow diluted Poly-L-Lysine solution to come to room temperature (18-26°C) before use.
- Place clean slides, a rack at a time, in diluted Poly-L-Lysine solution for 5 minutes.
- Drain slides and dry in 60°C oven for one hour or at room temperature (18-26°C) overnight.
How do you apply Poly-L-Lysine?
- Place glass coverslips in 6-well dish using special forceps for coverslips.
- Add 2 ml of poly-L-lysine (0.1% from Sigma, diluted 1:10 in sterile water) to each well.
- Wash 3x with sterile PBS.
- Place under the hood UV light 10-15 min to sterilize (remove cover).
- Plate cells as usual (use 2-2.5 ml for each well).
How do you dissolve Poly-L-Lysine?
To prepare a 0.05% stock, dissolve 5 mg of poly-l-lysine in 10 mL deionized water and filter-sterilize. Prepare aliquots of 1 mL and store them at −20°C for up to 1 yr.
What is the difference between L-lysine and D lysine?
The two enantiomers of lysine are L-lysine and D-lysine. The main difference between L-lysine and D-lysine is that L-lysine contains S configuration around the α-carbon while D-lysine contains the R configuration. Therefore, L-lysine is levorotatory, and D-lysine is dextrorotatory.
Is polylysine FDA approved?
Polylysine is an FDA approved natural homopolymer, which has been demonstrated to possess a wide spectrum antimicrobial activity with lesser toxicity to the human system.
Can I filter Poly-L-Lysine?
You can make a solution of known strength of poly-L-lysine aqueous solution and sterile filter using Millipore filtration unit in aseptic condition. Try 0.2micron syringe driven filters.
How do you dissolve poly-L-lysine?
How do you apply poly-L-lysine?
How do you apply Poly L-lysine?
Is polylysine a preservative?
Polylysine is a natural food preservative manufactured by JNC CORPORATION with the use of bio-technology. This naturally occurring substance is a chain polymer of the “essential amino acid” L-lysine, a protein that is essential for human health and development.
How do you dissolve poly lysine?
- Dissolve poly-d-lysine, (135kd molecular weight), in sterile water to 50ug/ml.
- Add 0.15ml/cm² of solution culture surface.
- Incubate 1-20 hours.
- Rinse 1 time and let dry.