Table of Contents
What is method of staining of volutin granule?
Volutin granules are mainly present in bacteria, algae,fungi and protozoa. These granules are stain by using Albert’s staining method.
Which staining is used to detect metachromatic granules?
Albert’s Stain A
Metachromatic staining is carried out where Albert’s Stain A is used as one of the stains and staining characteristic of organism is observed under microscope by using oil immersion lens. The metachromatic granules of Diphtheria bacilli stain black and the cytoplasm stains light green.
What stain will stain Corynebacterium diphtheriae?
Albert stain
Albert stain distinctly identifies metachromatic granules that are found in Corynebacterium diphtheriae.
What is the significance of volutin granule?
Volutin granules, sometimes termed metachromatic granules because of their colour reaction with the dyes used in light microscopy, contain polymerized inorganic phosphate, an energy-rich compound that acts as a reserve store of energy and of phosphate.
What type of stain is Albert stain?
differential stain
Albert’s stain was first described in 1920 and is a type of differential stain used for staining the volutin granules also known as metachromatic granules or food granules found in Corynebacterium diphtheriae.
What is Albert stain test?
Albert’s stain test is used to stain and observe metachromatic granules found in Corynebacterium diphtheriae. Albert stain is a kind of differential stain used for staining and identifying metachromatic granules. The granules appear purple-black when exposed to Albert’s stain and against the light green cytoplasm.
What is Volutin granules in microbiology?
Volutin granules are an intracytoplasmic storage form of complexed inorganic polyphosphate, the production of which is used as one of the identifying criteria when attempting to isolate Corynebacterium diphtheriae on Löffler’s medium.
What is stored substance in Volutin granules?
Volutin, or metachromatic granules, contains polymerized phosphate and represents a storage form for inorganic phosphate and energy. Many bacteria possess lipid droplets that contain polymeric esters of poly-β-hydroxybutyric acid or related compounds.
Why do we Albert stain?
Albert’s staining technique is a type of special staining technique since it is used to demonstrate a special structure in bacteria. It is chiefly used to demonstrate metachromatic granules found in Corynebacterium diphtheriae. This bacterium is responsible for the disease diphtheria.
How is the Golgi stain different from the Nissl stain?
The Nissl and Golgi stains are stains for differentiating cell structures. Nissl stains RNA so cell bodies stain blue/purple. Ribosomal RNA also picks up the stain. Golgi stains the entire cell membrane black, yet only stains ~1 in 500 cells.
Where are Volutin granules found?
Volutin granules can also be found in the cytoplasm of Saccharomyces, a genus of ascomycete fungi. They are characteristic for different species and depend on the age and condition of the culture.
What are bacteria inclusions?
Inclusion bodies are found in bacteria as particles of aggregated protein. They have a higher density than many other cell components but are porous. They typically represent sites of viral multiplication in a bacterium or a eukaryotic cell and usually consist of viral capsid proteins.
What is role of iodine in gram staining?
Gram’s iodine is used in Gram staining procedure to differentiate gram positive and gram negative organisms. Gram’s iodine acts as a mordant that causes the crystal violet to penetrate and adhere to the gram –positive organisms.
How do you stain Nissl bodies?
Procedure:
- Deparaffinize sections in xylene 2 or 3 changes at 10 minutes each.
- Stain in 0.1% cresyl violet solution for 3-10 minutes.
- Rinse quickly in distilled water.
- Differentiate in 95% ethyl alcohol for 2-30 minutes and check microscopically for best result.
- Dehydrate in 100% alcohol 2×5 min.
What is the difference between the Nissl stain and Golgi stain?
Lab 1B. Neuroanatomy: Nissl and Golgi stains The Nissl and Golgi stains are stains for differentiating cell structures. Nissl stains RNA so cell bodies stain blue/purple. Ribosomal RNA also picks up the stain. Golgi stains the entire cell membrane black, yet only stains ~1 in 500 cells.