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What is the 293 cell line?
293 [HEK-293] is a cell line exhibiting epithelial morphology that was isolated from the kidney of a human embryo. This cell line can be used in industrial biotechnology and toxicology research. It has applications in efficacy testing and viruscide testing.
Are HEK293 cells cancerous?
Thus, HEK293 is not a model for normal human cells; these cells are immortalized already by known oncogene but not malignant yet.
What is human embryonic kidney HEK293?
Human Embryonic Kidney (HEK) 293 is a cell line commonly used for BioPharma and Basic Medical research and therapeutic solutions. Generated in 1973, they are derived from human embryonic kidney cells and are popular, in part because of their reliable growth in culture and for their propensity for transfection.
Who HEK293?
Originating from a female fetus, HEK293 cells are today among the most used mammalian cell lines for a wide range of applications because of their ease of transfection as well as culture. Despite stemming from kidney tissue, HEK293 cells – time and time again – exhibited properties of immature neurons.
Why are HeLa cells so special?
In 1952, HeLa cells became the first human cell line that could grow and divide endlessly in a laboratory, leading scientists to label these cells “immortal”. The immortality of HeLa cells contributed to their adoption across the world as the human cell line of choice for biomedical research.
How was the 293T cell line created?
The 293T cell line was created in Michele Calos’s lab at Stanford by stable transfection of the HEK 293 cell line with a plasmid encoding a temperature-sensitive mutant of the SV40 large T antigen; it was originally referred to as 293/ tsA1609neo.
What are human embryonic kidney 293 cells?
Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture taken from a female fetus.
What is the new HEK 293T variant?
HEK 293T, a variant of the HEK 293 cell line, has been recently developed. This cell derivative carries SV40 large T antigen, enabling these cells to yield recombinant proteins in plasmid vectors that contain SV40 promoters.
What is the doubling time of HEK 293 cells?
Its doubling time is roughly about 34 to 36 hours. Human Embryonic Kidney 293 cells can be both grown as adherent or suspension but usually, these cells are cultured as an adherent monolayer. To keep HEK 293 cells alive, a high-glucose cell culture media is used. The media should be replaced in about 2-3 days.