Does Plakoglobin interact with actin?
β-Catenin and plakoglobin bind to the F-actin-binding protein α-catenin (6). α-Catenin may have a role in linking the cadherin-β-catenin complex to actin, but binding to β-catenin weakens the affinity of α-catenin for F-actin; it appears that α-catenin also serves to regulate actin dynamics and organization (8, 9).
Is Plakoglobin a cadherin?
The Molecular Biology of Cadherins Like beta-catenin, plakoglobin (gamma-catenin) utilizes its armadillo repeats to interact with binding partners (Fig.
What does Wnt protein do?
Wnt proteins are secreted, lipid-modified glycoproteins that allow for communication between cells. They regulate cell growth, function, differentiation, and cell death. Wnt proteins play a central role in bone development, modeling, and remodeling.
What activates Wnt signaling?
Wnt signaling begins when a Wnt protein binds to the N-terminal extra-cellular cysteine-rich domain of a Frizzled (Fz) family receptor. These receptors span the plasma membrane seven times and constitute a distinct family of G-protein coupled receptors (GPCRs).
How do I test my Wnt signal?
To measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay (Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from various sources including lentivirus-based reporters from the Nusse and Moon labs, through Addgene.
Where does the Wnt signaling work?
Wnt signaling plays a critical role in embryonic development. It operates in both vertebrates and invertebrates, including humans, frogs, zebrafish, C. elegans, Drosophila and others. It was first found in the segment polarity of Drosophila, where it helps to establish anterior and posterior polarities.
What is macula?
The macula is part of the retina at the back of the eye. It is only about 5mm across, but is responsible for our central vision, most of our colour vision and the fine detail of what we see. The macula has a very high concentration of photoreceptor cells – the cells that detect light.
What is plakoglobin made up of?
Plakoglobin (γ-catenin) is an armadillo protein that is present in the intracellular domain of desmosomes and fascia adherens junctions. Structurally, it is ~ 82 kD and consists of 12 armadillo repeats flanked by unique C-terminal and N-terminal domains.
What is the function of plakoglobin and beta-catenin?
Plakoglobin and beta-catenin: protein interactions, regulation and biological roles Beta-catenin can play different roles in the cell, including one as a structural protein at cell-cell adherens junctions and another as a transcriptional activator mediating Wnt signal transduction.
What is the role of plakoglobin in cell adhesion?
Plakoglobin is also an important regulator of cell adhesion and motility. For instance, the adhesive strength of desmosomes was shown to be downregulated by phosphorylation of plakoglobin following activation of the epidermal growth factor receptor (EGFR).