Table of Contents
How does an LCMS work?
The mass spectrometer for an LC/MS/MS system has an ionization source where the LC column effluent is nebulized, desolvated and ionized creating charged particles. These charged particles then migrate under high vacuum through a series of mass analyzers (quadrupole) by applying electromagnetic fields.
What is Orbitrap mass analyzer?
In mass spectrometry, Orbitrap is an ion trap mass analyzer consisting of an outer barrel-like electrode and a coaxial inner spindle-like electrode that traps ions in an orbital motion around the spindle.
What is difference between ion trap and Orbitrap?
The Orbitrap is a modified Kingdon ion trap that consists of two endcap electrodes and a central “spindle” electrode. A DC current applied to the Orbitrap spindle electrode results in a high static voltage between the endcap electrodes.
Why Agilent 6490 triple quadrupole LC/MS system?
The Agilent 6490 Triple Quadrupole LC/MS System incorpo- rates iFunnel technology to give new levels of sensitivity for the most challenging quantitative analyses in pharmaceuti- cal, clinical, food safety, and environmental applications. The quantitative power of the 6490 Triple Quadrupole is enhanced by an extended linear dynamic range.
What is ifunnel technology on the 6490 triple quadrupole?
Agilent’s iFunnel Technology which is available on the Agilent 6490 Triple Quadrupole consists of the Agilent Jet Stream technology, the Hexabore Capillary and the Dual Ion Funnel. The Agilent Jet Stream technology is discussed below.
Why HPLC-Chip/MS with 6490 triple quad for peptide quantitation?
For ultra- sensitive results from limited samples, the HPLC-Chip/MS with 6490 Triple Quad offers a robust alternative to nanofl ow LC/MS for peptide quantitation. The new 6490 Triple Quadrupole mass spectrometer with iFunnel technology redefi nes sensitivity, robustness and dynamic range for the most challenging quantitative applica- tions.
How many pumps does the 6490 triple quadrupole have?
Pumping system 6490 Triple Quadrupole The 6490 has two roughing pumps and a three-stage turbo pump for the first five vacuum stages. The two roughing pumps are needed to decrease the pressure in the Dual Ion Funnel.