Table of Contents
What are anti GFP antibodies?
Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin.
Which antibody is tagged with GFP?
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms.
Does GFP antibody recognize YFP?
GFP antibody (ab6556) is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.
Is GFP an antigen?
The target antigen refers to green fluorescent protein (GFP), commonly used as an epitope tag for recombinant proteins for detection via immunofluorescence, flow cytometry or western blot.
What is the purpose of GFP?
Biologists use GFP as a marker protein. GFP can attach to and mark another protein with fluorescence, enabling scientists to see the presence of the particular protein in an organic structure. Gfp refers to the gene that produces green fluorescent protein.
Is GFP immunogenic in mice?
These findings suggest that eGFP is immunogenic in Balb/c mice whereas it is only slightly immunogenic in C57BL/6 mice….Table 1.
| GFP Immunogenicity | GFP Cytotoxicity |
|---|---|
| Diminished immunogenicity by immunosuppressant [8] | Cardiac cytotoxicity [38] |
Can I use mCherry and GFP?
Due to the spectral properties of GFP and mCherry, they are considered an ideal combination for co-localisation and co-expression experiments.
How large is GFP RFP in kDa?
RFP is approximately 25.9 kDa. The excitation maximum is 558 nm, and the emission maximum is 583 nm. The first fluorescent protein to be discovered, green fluorescent protein (GFP), has been adapted to identify and develop fluorescent markers in other colors.
How many BP is GFP?
730 bp
The GFP cDNA consists of 730 bp, which encode a 238 amino acid protein with a molecular weight of 27 kD (2). Wild-type GFP emits a vibrant green fluorescence upon exposure to blue light (450-490 nm). The signal is detectable by fluorescence microscopy and fluorescence-activated cell sorting (FACS) (3).
Why is YFP green?
Because the emission passband (centered at 535 nanometers) overlaps the green spectral region, fluorescence from YFP appears visually to be green, rather than yellow.