What is laser capture microdissection used for?
Laser capture microdissection is a powerful technique that enables oncology researchers to compare molecular profiles of tumor tissue to surrounding nontumor cells.
What is a microdissection instrument?
Laser Microdissection LMD. Laser Microdissection, also known as LMD or LCM (Laser Capture Microdissection), is a contact- and contamination-free method for isolating specific single cells or entire areas of tissue from a wide variety of tissue samples.
Who invented laser capture microdissection?
Dr. Emmert-Buck
Today, there are two general classes of laser microdissection: infrared (IR) laser-capture system and ultraviolet (UV) laser-cutting system. The laser-capture technique was developed in the 1990s by Dr. Emmert-Buck and colleagues at the National Institutes of Health (Bethesda, MD, USA).
What is LCM pathology?
General Toxicologic Pathology Laser capture microdissection (LCM) provides a topographical bridge to genomics and proteomics methodologies and can be utilized to overcome the problems arising from use of heterogeneous cell populations to study the effects of toxicants.
What is the purpose of microdissection of tissue sections in oncology?
Tissue microdissection is a laboratory method that is used to procure specific cells or cell populations from a histology slide under direct microscopic visualization.
What is macro dissection?
macrodissection (countable and uncountable, plural macrodissections) dissection without the use of microscopes.
What is the role of LCM in drilling fluids?
Fibrous LCM can be added to the drilling fluid and pumped downhole to help in reducing losses & maintaining circulation if there is a lost circulation into fractures or highly permeable zones. Cedar fiber (wood fiber). Sawdust (wood fiber).
How are LCM pills made?
LCM pills are usually prepared using mud from the active circulating system. However, this will produce a higher density pill once all the LCM products have been mixed and this might increase the hydrostatic pressure of the fluid and the loss rate to the formation when pumped downhole.
How do you use LCM to seal a pipe?
Firstly, Spot a viscous pill comprising 30 ppb to 40 ppb fine and medium grade LCM (Nut Plug, Mica and K.Seal) across the suspected loss zone. Pull the drill string up into the casing shoe to minimize the potential for differential pipe sticking while leaving the LCM pill to soak in the loss zone for a few hours.