Table of Contents
What is NanoDrop technology?
NanoDrop microvolume technology employs a sample retention system that relies on the surface tension properties of the sample being measured to form a liquid column. It is essential that the sample makes contact with the upper and lower optical measurement surfaces for proper column formation.
What kind of spectrophotometer is a NanoDrop?
The NanoDrop® ND-1000 UV-Vis Spectrophotometer is a spectrophotometer which enables highly accurate analyses of 1 ul samples for DNA, RNA, protein, pigments, whole uv-visible (220-700nm) spectrum and cell density tests with remarkable reproducibility.
What is the purpose of the NanoDrop spectrophotometer we will use?
A NanoDrop spectrophotometer is a common lab instrument that can measure the concentration of DNA, RNA, and protein in a 2-µL drop on a pedestal. Such a small sample volume means less prep and cleanup time and enables you to measure several samples in under a minute compared to using a traditional 1-cm cuvette.
What is the principle of NanoDrop spectrophotometer?
Principle: It operates on Beer’s law. The sample retention system in NanoDrop Spectrophotometry employs surface tension to hold the sample in place between two optical fibers. This enables the measurement of very highly concentrated samples without the need for dilutions.
What is difference between NanoDrop and spectrophotometer?
Another useful feature of the NanoDrop is the ability to measure CyDye incorporation into microarray probes. Using the NanoDrop to check probe quality is now a standard procedure in our lab. Unlike other small spectrophotometers, the NanoDrop is able to scan samples throughout the 220-750nm range.
What are the advantages of using NanoDrop technology?
The main advantage the Nanodrop has over the Qubit is the ability to measure the purity of the sample. The 260/280 and 260/230 ratios give an indication of how pure the sample is from protein and salt contaminants.
How is NanoDrop different from spectrophotometer?
How does a nano spectrophotometer work?
The NanoDrop 3300 model allows fluorescence analysis of micro-volume samples. With the arm open, a fluorescent sample is pipetted directly onto the pedestal. After the arm is closed, a sample column is formed and held in place by surface tension during the fluorescence measurement.