Table of Contents
What is a slide staining rack?
Staining racks hold multiple microscopic slides simultaneously for the most efficient staining processing. Although accommodating different specific number of slides, all models ensure the safe handling of the fragile glassware.
What is the purpose of staining rack?
The staining rack is produced from polyoxymethylene (POM) and is designed for the simultaneous staining of up to 25 microscope slides. The staining dish is designed for use with our staining rack and features a lid that holds up to 28…
How do you stain a slide at home?
To begin, use a 1:1 dilution ratio of dye solution to water. Mount your specimen on a glass slide. For a simple wet mount, apply a drop of water to the slide and carefully place the specimen on the wet portion. Apply a few drops of diluted dye to the sample.
What is staining rack made of?
The staining rack is produced from polyoxymethylene (POM) and is designed for the simultaneous staining of up to 25 microscope slides.
Is Coplin jar used in staining?
Coplin jars can be used for pre-staining with and alcohol buffer. Slides are held vertically for easy manipulation. A Coplin jar can also serve as a preparation chamber for micro-thin-layer chromatography.
What is the function of glass slide?
A glass slide is a thin, flat, rectangular piece of glass that is used as a platform for microscopic specimen observation. A typical glass slide usually measures 25 mm wide by 75 mm, or 1 inch by 3 inches long, and is designed to fit under the stage clips on a microscope stage.
Why is using methylene blue as a stain necessary?
Methylene Blue is a popular alkaline stain used to view microscopic life in brilliant color. It helps make cells show up against their background, where their shape can help you determine what they are (their morphology).
What is a Coplin jar?
Definition of Coplin jar : a covered glass vessel that is rectangular in cross section and grooved inside for holding microscope slides vertical during processing.
What is the purpose of Coplin jar?
How do you make a glass slide hydrophobic?
Silanization of glass microscope slides using hexamethyldisil Azane (HMDS) can make slides hydrophobic dust repellent. Simply place the separated slides in a container like a wide mouth glass jar with an aluminium or Teflon lined cap.
Why after slide has stained and dried not be necessary to use a cover slip?
Wet slides will use a cover slip or cover glass, a very thin square piece of glass (or plastic) that is placed over the sample drop. Without the cover in place, surface tension would cause the droplet to bunch up in a dome.
Why is it important to wash the glass slides prior to staining?
The bacteria need to be firmly attached to the slide so they are not washed off during the staining procedures. All procedures that attach the bacteria to the slide result in some morphological changes.
Why do we heat fix slides before staining?
Heat fixing kills the bacteria in the smear, firmly adheres the smear to the slide, and allows the sample to more readily take up stains.
What is a substitute for methylene blue?
Gentian violet also appears to be a better alternate to methylene blue.
How do you stain a slide with methylene blue?
Ⅴ. How to stain with methylene blue
- After performing the bacterial smear (smearing, fixing)
- Place the slide on a staining rack and flood it with methylene blue.
- Gently wash the slide with distilled water, drain off excess water, blot (do not rub) with absorbent paper and allow slides to air dry completely.