What is EHA105?

What is EHA105?

The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.

What is LBA4404?

The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants. LBA4404 contains a rifampicin resistance gene (rif). LBA4404 strain also contains a octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.

What is the selection for Agrobacterium strain LBA4404?

You can use antibiotic selection to test it: LBA4404: rifampicin and streptomycin. Streak your bacteria on LB + rifampicin + streptomycin.

How do you make Agrobacterium competent cells?

Competent cells are made by streaking the master stock onto a rifampicin plate, growing a single colony in a large culture, and then washing the pellet of cells several times with 10% glycerol and flash freezing in liquid nitrogen.

How do you identify Agrobacterium tumefaciens?

A. tumefaciens can be effectively isolated for identification from gall tissue, soil or water. Optimal gall tissue for isolation is white or cream-colored from a young, actively growing gall. The gall should be washed or surface sterilized using 20% household bleach, and rinsed several times in sterile water.

Which is associated with Agrobacterium tumefaciens?

Biology. Assertion: Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated with the roots of all cereal and pulse crops. Reason: A gene incorporated in the bacterial chromosomal gene gets automatically transferred to the crop with which the bacterium is associated.

What is the significant features of Agrobacterium LBA4404?

LBA4404 is a widely used strain for plant transformation. This product contains competent cells for transformation by electroporation. Using A. tumefaciens and the binary vector method, you can transform various plants for infection (transfection) experiments.

How do you make glycerol stock in Agrobacterium?

Prepare a glycerol stock of the remaining culture from step 3 by mixing 600 µL Agrobacterium culture with 300 µL sterile 30% glycerol in a microcentrifuge tube. This stock can be stored at -70°C indefinitely. 6.

Where can I buy Agrobacterium tumefaciens?

Agrobacterium tumefaciens is cosmopolitan in distribution, affecting dicotyledonous plants in more than 60 different plant families. Crown gall can be found most often on stone fruit and pome trees as well as brambles and several species of ornamental plants.

What is dahlia gall?

What is crown gall? Well, it’s a pretty gross-looking disease that affects not just dahlia tubers, but fruit trees, roses, and poplar trees, as well as many others. Essentially it’s a cancer–it causes a tumor to grow on the roots or trunk of plants and causes the plant cells to replicate endlessly.

What is Agrobacterium tumefaciens and Bacillus thuringiensis?

tumefaciens has enabled this bacterium to be used as a tool in plant breeding. Any desired genes, such as insecticidal toxin genes (see Bacillus thuringiensis) or herbicide-resistance genes, can be engineered into the bacterial DNA and thereby inserted into the plant genome.

Who discovered Agrobacterium tumefaciens?

Abstract: The causative organism of the crown gall disease of plants, Agrobacterium tumefaciens, was discovered by Smith and Townsend (Smith and Townsend, 1907).

What is Agrobacterium tumefaciens used for?

Agrobacterium tumefaciens is a gram-negative bacterium that uses horizontal gene transfer to create tumors in plants. Agrobacterium tumefaciens has been extensively used as major agent for the production of transgenic plants in a wide variety of plant species (Hooykaas, 1989).

Can we autoclave acetosyringone?

Acetosyringone should NOT be autoclaved. This is a sterile solution of Acetosyringone in DMSO.

What is streptomycin injection used for?

Streptomycin injection is used to treat moderate to severe bacterial infections in many different parts of the body. Streptomycin belongs to the class of medicines known as aminoglycoside antibiotics. It works by killing bacteria or preventing their growth.

What is mycobacterial growth in the presence of streptomycin?

Mycobacterial growth in the presence of drug ≥1% of the control indicates resistance. The radiometric broth method employs the BACTEC 460 machine to compare the growth index from untreated control cultures to cultures grown in the presence of 6.0 mcg/mL of streptomycin.

What is the dosage of streptomycin 500 mg for enterococcal infection?

If the patient is over 60 years of age, the dosage should be 500 mg b.i.d. for the entire 2-week period. Enterococcal endocarditis: Streptomycin in doses of 1 g b.i.d. for 2 weeks and 500 mg b.i.d. for an additional 4 weeks is given in combination with penicillin.

When is it safe to stop treatment with streptomycin for tuberculosis?

Therapy with streptomycin may be terminated when toxic symptoms have appeared, when impending toxicity is feared, when organisms become resistant, or when full treatment effect has been obtained. The total period of drug treatment of tuberculosis is a minimum of 1 year; however,…