Table of Contents
What is the mechanism of ion exchange chromatography?
Ion-exchange chromatography uses column packing material that has charge-bearing functional groups attached to a polymer matrix. The mechanism in this type of chromatography is the exchange of sample ions and mobile-phase ions with the charged group of the stationary phase.
What does ion exchange chromatography measure?
Ion chromatography is used for water chemistry analysis. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range.
Which ion exchanger is used in ion exchange chromatography?
Alkylated amino groups for anion exchangers and carboxy, sulfo as well as phosphato groups for cation exchangers are the most common functional groups used on ion exchange chromatography supports [14]. Sulfonic acid exchangers are known as strong acid type cation exchangers.
What are the advantages of ion exchange chromatography?
Advantages of ion exchange chromatography It is one of the most efficient methods for the separation of charged particles. It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids.
How many types of ion exchange chromatography are there?
two types
The two types of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is used when the molecule of interest is positively charged.
What is ion exchange chromatography (ion chromatography)?
Ion exchange chromatography (or ion chromatography) is a process that allows the separation of ions and polar molecules based on their affinity to ion exchangers.
What are the advantages and disadvantages of ion exchange chromatography?
Advantages of ion exchange chromatography It is one of the most efficient methods for the separation of charged particles. It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids.
What is ion exchange used for in chemistry?
It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids. Ion exchange is used for both analytical and preparative purposes in the laboratory, the analytical uses being the more common. Only charged molecules can be separated.
What is the role of chromatography in forensic science?
Chromatography is only one part of an effective forensic investigation. Whilst it separates them, it still needs a way to effectively detect and categorize the compounds. Some compounds can be easily mistaken for other similar compounds as well. An effective method that can be coupled with chromatography techniques is mass spectrometry.