Table of Contents
What is a biosafety level 2 laboratory?
BSL–2. This biosafety level covers laboratories that work with agents associated with human diseases (i.e. pathogenic or infections organisms) that pose a moderate health hazard.
What is biosafety level 2 Plus?
Biosafety Level 2 Plus (BSL-2+) is the common term for laboratories where work with microorganisms is conducted in a BSL-2 laboratory with biosafety practices and procedures that are typically found at BSL-3.
What is the difference between BSL 2 and BSL-3?
This is provided to demonstrate that BSL-2 is largely achieved through sound lab safety practices and BSL-3 is achieved through the addition of facility features and equipment designed for containment of aerosols to a foundation of sound lab safety practices.
What PPE is always required in a biosafety level 2 bl2 laboratory?
Protective laboratory coats, gowns, smocks, or uniforms designated for lab use must be worn when working with BSL2 materials. This protective clothing is removed and left in the laboratory before leaving for non-laboratory areas (e.g., cafeteria, library, administrative offices).
What is the type of organisms that initiate the use of biological safety level 2?
Biosafety level two would cover work with agents associated with human disease, in other words, pathogenic or infectious organisms posing a moderate hazard. Examples are the equine encephalitis viruses and HIV when performing routine diagnostic procedures or work with clinical specimens.
What is a Category 3 laboratory?
Containment level 3 (CL 3) is used for work with high risk biological agents and hazards, genetically modified organisms, animals and plants.
What are the minimum requirements for a BSL-2 biological safety cabinet?
Biosafety Level 2 (BSL-2) BSL-2 laboratories must also have access to equipment that can decontaminate laboratory waste, including an incinerator, an autoclave, and/or another method of decontamination, depending on the biological risk assessment. Access to the laboratory is restricted when work is being conducted.
Which BSL level is for lethal agents for which there are no vaccines or other countermeasures?
Biosafety Level 4 (BSL-4) BSL-4 laboratories are used to study infectious agents or toxins that pose a high risk of aerosol-transmitted laboratory infections and life-threatening disease for which no vaccine or therapy is available.
What are the personal protective equipment requirements for a biosafety level 2 lab?
Standard BSL-2 PPE consists of a dedicated lab coat, gloves, and eye protection. Other PPE may be required (see EH&S PPE Assessment Guide). Do not wear PPE in public areas.
What are some pieces of personal protective equipment we use in our BSL-2 lab?
BSL-2
- Gowns.
- Gloves.
- Face and eye protection as needed.
Which of the following materials must be handled at BSL-2?
Gloves and Eye Protection Gloves must be worn while working at the BSL2 level. necessary.
What are the four hazard groups?
4 Types of Workplace Hazards
- Physical Hazards. Physical hazards are the most common type of workplace hazards.
- Biological Hazards.
- Ergonomic Hazards.
- Chemical Hazards.
What is a Level 1 lab?
Biosafety level 1 (BSL-1) is suitable for work with well-characterized agents which do not cause disease in healthy humans. In general, these agents should pose minimal potential hazard to laboratory personnel and the environment. At this level, precautions are limited relative to other levels.
How often does a biosafety cabinet need to be certified?
annually
BSCs must be certified when installed, whenever they are moved and at least annually [29 CFR 1910.1030(e)(2) (iii)(B)]. Employers should ensure that a risk assessment has been completed and approved for the work to be conducted and to identify the class and type of BSC needed for the operation or procedure.
What are the three primary routes of transmission at BSL 2?
inhalation, 2. direct contact with skin or mucous membranes, 3. ingestion, and 4.
What is an example of a Biosafety Level 2 agent?
The agents require Biosafety Level 2 containment. Examples of BSL-2 organisms are: Mycobacterium, Streptococcus pneumonia, Salmonella choleraesuis. BSL-3 risk group contains biological agents that usually cause serious disease (human, animal or plant) or that can result in serious economic consequences.
What is BSL-2 biosafety level?
Biosafety Level 2 (BSL-2) is suitable for experiments involving agents of moderate potential hazard to personnel and the environment.
What are the different levels of biosafety?
Biological safety levels are ranked from one to four and are selected based on the agents or organisms on which the research or work is being conducted. Each level up builds on the previous level, adding constraints and barriers A very specialized research laboratory that deals with infectious agents is the biosafety lab.
What are the laboratory requirements for coms-approved projects?
Minimum Biosafety Level 2+ Laboratory Requirements for COMS-Approved Projects. Additional stipulations may be listed in your approval letter. Biosafety Level 2+ (BL2+) builds upon Biosafety Level 2 (BL2). All requirements of BL2 are also required for BL2+, with the addition of the following:
What are the Biosafety procedures in a laboratory?
Biosafety procedures are incorporated into standard operating procedures or in a biosafety manual adopted or prepared specifically for the laboratory by the laboratory director. Personnel are advised of special hazards and are required to read and follow instructions on practices and procedures.