Table of Contents
What is the use of electronic pipette?
Generally speaking, an electronic pipet should be used for all applications that border on automation. Mechanical pipetting is used most when you have a limited number of different samples, with fewer repetitive steps. Mechanical pipets can be used for transfer of liquids or dilutions, but not for dispensing.
How many types of pipetting are there?
The five grades of pipettes include disposable/transfer, graduated/serological, single-channel, multichannel, and repeat pipette.
How do you clean an electronic pipette?
Open the pipette, immerse the parts in a detergent solution, such as Deconex® 12 Basic. Rinse well with distilled water and allow to dry. DNA can be eliminated by immersing pipette parts in at least 3% (w/v) sodium hypochlorite for at least 15 minutes (2,3). Rinse well with distilled water and allow to dry.
Which type of pipette is most accurate?
volumetric pipette
While there are many different types of pipette are available, it’s important to remember that certain pipettes offer more accuracy than others. The volumetric pipette remains the world’s most accurate.
How much does electronic pipette cost?
$1000 to $1500
Electronic pipettes are usually two to three times more expensive than manual pipettes. If a single-channel manual pipette costs around $300, an electronic pipette will cost you anywhere from $1000 to $1500.
Which pipette is most accurate?
Volumetric pipettes
Volumetric pipettes are considered the most accurate, with the capacity to measure up to four significant figures.
How do I choose a pipette?
As a rule of thumb, always choose the smallest pipette capable of handling the required volume. This is important because accuracy decreases when the set volume is close to the pipette’s minimum capacity. For example, if you dispense 50 µl using a 5,000 µl pipette, you will get rather poor results.
What are some common pipetting errors?
Common pipetting errors include:
- Failure to Pre-Wet Pipette Tip.
- Disregarding Temperature.
- Tip Wiping.
- Choosing the Wrong Pipetting Mode.
- Working Too Quickly.
- Pipetting at an Angle.
- Using the Wrong Pipette Tips.
Why do we use micropipettes?
A micropipette is a common yet an essential laboratory instrument used to accurately and precisely transfer volumes of liquid in the microliter range.