Table of Contents
What is widefield microscopy used for?
Widefield microscopes are excellent for producing 2D images of specimens as the entire field can be captured at once. The amount and localisation of specific fluorescent molecules can be seen with fast temporal resolution. Processes in live cells, such as neuronal signalling, can be measured in real time.
What are wide field images?
Any microscope technique where the entire sample is exposed to light is known as ‘widefield’ imaging. The counterpart to widefield is confocal, where pinholes are used to block most of the light to and from the sample.
What is the purpose of epifluorescence microscopy?
Why is epifluorescence microscopy useful? Epifluorescence microscopy is widely used in cell biology as the illumination beam penetrates the full depth of the sample, allowing easy imaging of intense signals and co-localization studies with multi-colored labeling on the same sample.
What is Descanning microscopy?
Descanning – The process of allowing light emitted by the specimen in confocal microscopy to be collected by the objective and retrace its path back through the scanning mirrors to the dichromatic mirror.
What does wide field mean?
Widefield definition (microscopy) In which a whole two-dimensional image is acquired simultaneously using a wide-area detector array. adjective.
What is fluorescein angiography used for?
This test is done to see if there is proper blood flow in the blood vessels in the two layers in the back of your eye (the retina and choroid). It can also be used to diagnose problems in the eye or to determine how well certain eye treatments are working.
What can you see with fluorescence microscopy?
In the life sciences fluorescence microscopy is a powerful tool which allows the specific and sensitive staining of a specimen in order to detect the distribution of proteins or other molecules of interest. As a result, there is a diverse range of techniques for fluorescent staining of biological samples.
What is widefield imaging in microscopy?
Any microscope technique where the entire sample is exposed to light is known as ‘widefield’ imaging. The counterpart to widefield is confocal, where pinholes are used to block most of the light to and from the sample. This article will discuss widefield imaging and the most widely-used widefield techniques in microscopy.
What is a wide field fluorescence microscope?
Wide-field fluorescence microscopy. The Cell Imaging Center houses two wide-field microscopes: the Olympus IX81 and the Zeiss AxioObserver. A wide-field microscope is a type of fluorescence microscope in which the entire specimen is illuminated by a white light source, usually a mercury arc lamp.
What are the limitations of widefield microscopy?
The emitted fluorescence can scatter, making the image appear blurry. This makes widefield microscopy sometimes unsuitable for producing 3D images. Fluorescence deconvolution microscopy and structured illumination microscopy can be solutions to these problems.
What kind of microscopes are used in the cell imaging center?
The Cell Imaging Center houses two wide-field microscopes: the Olympus IX81 and the Zeiss AxioObserver. A wide-field microscope is a type of fluorescence microscope in which the entire specimen is illuminated by a white light source, usually a mercury arc lamp. Optical filters are used to generate excitation light of specific wavelengths.