Table of Contents
What is Haelll restriction enzyme?
HaeIII is one of many restriction enzymes (endonucleases) a type of prokaryotic DNA that protects organisms from unknown, foreign DNA. It is a restriction enzyme used in molecular biology laboratories. It was the third endonuclease to be isolated from the Haemophilus aegyptius bacteria.
What is HindIII recognition sequence?
Hind III recognizes the sequence A/AGCTT and gene- rates fragments with 5´-cohesive termini (1). Compatible ends The enzyme is not known to have compatible ends. Isoschizomers The enzyme is not known to have isoschizomers. Hind III is inhibited by 6-methyladenine and 5-methyl- cytosine as indicated (*).
Where can I find more information about BSA-free Restriction enzymes?
Find more details at www.neb.com/BSA-free. Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence. An E. coli strain that carries the BsmI gene from Bacillus stearothermophilus NUB 36 (N. Welker).
What is an IIS restriction enzyme?
Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence. An E. coli strain that carries the BsmI gene from Bacillus stearothermophilus NUB 36 (N. Welker). One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 65°C in a total reaction volume of 50 µl.
Which restriction enzymes recognize gaatgc (1/-1) ^ sites?
Thermo Scientific Mva1269I (BsmI) restriction enzyme recognizes GAATGC (1/-1)^ sites and cuts best at 37°C in R buffer (isoschizomers: BsaMI, BsmI, PctI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
What is the best restriction enzyme for DNA digestion?
Note: Also available as a FastDigest enzyme for rapid DNA digestion. Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions.