Table of Contents
What companies produce Taq polymerase?
Suppliers of Taq DNA Polymerases
| Company | Catalog # | |
|---|---|---|
| Ambion (800) 888-8804 www.ambion.com | 2052 | SuperTaq (manufactured by Enzyme Technologies) |
| 2054 | SuperTaq Plus Polymerase | |
| 2056 | ||
| Amersham Pharmacia Biotech (800) 526-3593 www.apbiotech.com | 27-0799-01 | Taq DNA Polymerase |
How much does Taq polymerase cost?
With those caveats in mind, the average price for standard Taq polymerases without volume discounts can range around $0.57 per reaction or $0.32 per unit.
What is Qiagen Q solution?
Q-Solution changes the melting behavior of DNA and will often improve a suboptimal PCR caused by templates that have a high degree of secondary structure or high GC-contents.
What is the product of Taq polymerase?
Taq polymerase generates single 3′-A overhangs with its terminal transferase activity. These can be used for cloning PCR products into TA cloning vectors.
Is Taq polymerase expensive?
Though Taq DNA polymerase has been cloned since 25 years ago [3] and is not expensive now, the expense on the enzyme is also astonishing to some labs that need to do large amount of PCRs to analyze the polymorphism of molecular markers for construction of a genetic map, positional cloning, and evolutionary analysis etc …
Where is Taq polymerase made?
Taq polymerase was identified from T. aquaticus isolated from Yellowstone National Park in Montana, USA.
How big is Taq?
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity.
What does Q solution do?
What is hot start Taq polymerase?
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature.
Where does Taq polymerase come from?
function in polymerase chain reaction …a heat-stable DNA polymerase called Taq, an enzyme isolated from the thermophilic bacterium Thermus aquaticus, which inhabits hot springs.
How do you get Taq polymerase?
Taq polymerase is the heat-stable (thermostable) DNA polymerase extracted from the thermophilic bacteria Thermus aquaticus. Its predominant function is in the polymerase chain reaction (PCR) technique, where it automates the repetitive step of amplifying specific DNA sequences.
How is Taq polymerase made?
Taq DNA polymerase is an enzyme essential in performing Polymerase Chain Reaction (PCR) which has recently become a basic technology in research and diagnostic laboratories. In order to reduce the cost of research work in Thailand, recombinant Taq DNA polymerase was locally produced from pTaq cloned in E. coli.
How is Taq polymerase produced?
What is Gc enhancer?
GC PCR Enhancer Kit is an optimized Horse-Power™ Taq DNA Polymerase (with TruePure™ dNTPs). It is a pure, versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus.
How does Q solution work PCR?
What is GC rich PCR?
GC-rich DNA sequences are inherently more stable than sequences with a low GC content. For PCR, this means that the higher the GC content, the higher the melting point of the DNA. The increased stability of GC-rich DNA sequences is, contrary to popular belief, not primarily because of the hydrogen bonds.
Where was Taq polymerase found?
Yellowstone National Park
Taq polymerase was identified from T. aquaticus isolated from Yellowstone National Park in Montana, USA. The report was published by Chien et al. (1976) as her Master’s course study.
Why is Taq important to industry?
The reason that the taq polymerase has become so significant to biotechnological processes is because of the resistance of the enzyme to heat. A molecular biology technique known as the polymerase chain reaction relies upon the exposure of DNA to heat in order to separate the two strands of the double helix.
What is Taq polymerase and why is it important?
Properties:
Why is Taq polymerase so important?
Low Accuracy: The most important limitation of Taq polymerase is that it’s not the most accurate polymerase enzyme.
What are the unique properties of Taq polymerase?
Antibodies,Monoclonal
How did Taq polymerase acquire its name?
Taq. polymerase. Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by Chien et al. in 1976. Its name is often abbreviated to Taq or Taq pol.