Table of Contents
What does cell mask stain?
What dyes are used to make the CellMask stains? The proprietary fluorescent dyes in the CellMask stains are general cytoplasmic stains. They are not found to bind to any specific cellular component.
What is DAPI color?
blue-
DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA.
Why is it called plasma membrane?
Why it is called a plasma membrane? The cell contains a protoplasm (or simply plasm), which is the semi-fluid living matter. This living matter or plasm is contained inside a biological membrane referred to as the plasma membrane.
Is DAPI a dye?
DAPI (4′,6-Diamidino-2-Phenylindole, dihydrochloride) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining, and flow cytometry.
What does Hoechst stain?
Hoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. This stain is commonly used in combination with 5-bromo-2′-deoxyuridine (BrdU) labeling to distinguish the compact chromatin of apoptotic nuclei, to identify replicating cells and to sort cells based on their DNA content.
What are cell surface and membrane dyes?
Cell Surface & Membrane Dyes. Biotium offers two new classes of dyes for covalent labeling of the cell surface that are unique in that they can withstand fixation and permeabilization for subsequent IF staining. CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells.
Can cyanine dyes be used to label Plasma membranes?
Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods.
What is fix membrane stain?
CellBrite™ Fix Membrane Stains are a new class of fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. They are unique among membrane stains in that they can withstand both fixation and detergent permeabilization. Available with green, red, and far-red fluorescence.
What is the purpose of staining the plasma membrane?
These stains may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The stain survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies. For Research Use Only.