Table of Contents
How does oligonucleotide synthesis work?
In solid-phase synthesis, an oligonucleotide being assembled is covalently bound, via its 3′-terminal hydroxy group, to a solid support material and remains attached to it over the entire course of the chain assembly.
How do DNA synthesizers work?
Automated DNA Synthesizers: Introduction During DNA synthesis, the DNA strands are copied in a cell before cell division or replication. DNA synthesizer uses one strand as a blue print to build DNA molecules. Subsequently, it uses DNA protein synthesis to repair any discrepancies in the strand.
What is oligo mixture?
Standard “wobbles” or “mixed bases” are mixtures of two or more different bases at a given position within the sequence. Wobbles are often incorporated into oligonucleotide probes designed to hybridize to an unknown gene that encodes a known amino acid sequence.
What is the purpose of the oligonucleotides in the reaction?
Oligonucleotides made up of 2′-deoxyribonucleotides are the molecules used in polymerase chain reaction (PCR). These are referred to as primers and are used to massively amplify a small amount of DNA.
Why do we need DNA synthesis?
DNA replication needs to occur because existing cells divide to produce new cells. Each cell needs a full instruction manual to operate properly. So the DNA needs to be copied before cell division so that each new cell receives a full set of instructions!
Are oligos and primers the same thing?
What are oligodeoxynucleotides?
CpG-oligodeoxynucleotides (ODN) is a short synthetic DNA containing unmethylated CpG motifs, highly prevalent in bacterial DNA, and recognized by Toll-like receptor 9 (TLR9), which is expressed by a variety of cells in the immune system.
What is oligo annealing?
Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences. Heat breaks all hydrogen bonds, and cooling allows new bonds to form between the sequences.
Can you create DNA?
Because artificial gene synthesis does not require template DNA, it is theoretically possible to make a completely synthetic DNA molecule with no limits on the nucleotide sequence or size.
What is an oligo dT primer?
Description. Oligo (dT)18 Primer is single-stranded sequence of deoxythymine (dT), used for priming reactions catalysed by reverse transcriptase. The transcript is primed in the poly(A) tail of mRNA molecules.
What synthesizers do you use to synthesize oligos?
For nonstandard oligos, we use synthesizers optimized for enhanced flexibility. These synthesizers facilitate the addition of complex modifications, such as fluorophores, quenchers, linkers, spacers, and modified bases. Though our synthesis platforms vary in speed and flexibility, they all use solid-phase synthesis to produce an oligo.
How is an oligo synthesized?
Though our synthesis platforms vary in speed and flexibility, they all use solid-phase synthesis to produce an oligo. This means oligos will be tethered to a solid surface while they are being made. At IDT, we engineer our solid supports in-house, and we can easily optimize their properties to accommodate oligo synthesis at different scales.
Why do we use solid phase synthesis for oligos?
These synthesizers facilitate the addition of complex modifications, such as fluorophores, quenchers, linkers, spacers, and modified bases. Though our synthesis platforms vary in speed and flexibility, they all use solid-phase synthesis to produce an oligo. This means oligos will be tethered to a solid surface while they are being made.
What are the advantages of solid phase synthesizers?
These synthesizers facilitate the addition of complex modifications, such as fluorophores, quenchers, linkers, spacers, and modified bases. Though our synthesis platforms vary in speed and flexibility, they all use solid-phase synthesis to produce an oligo.