Table of Contents
What is a normal peripheral blood smear?
A normal peripheral blood smear indicates the appropriate appearance of red blood cells, with a zone of central pallor occupying about 1/3 of the size of the RBC.
What is the characteristic of good blood smear?
A well made blood smear. The classic blood smear has a thumb print appearance. A well developed feathered edge is apparent. The dense body of the smear takes up most of the slide and there is a thin counting area which blends with the feathered edge and the dense body.
How do you describe a peripheral smear?
A peripheral blood smear test shows how your blood cells and platelets look under a microscope. Looking through a microscope, your healthcare provider may see: Changes in your blood cell and platelet size and shape may be a sign of a blood disorder or blood cancer.
What is a normal hemo slide number?
14.5–24.5 g/dL or 145–245 g/L. In general, a normal hemoglobin level is about one-third the value of the hematocrit. Red blood cell indices footnote 1. Mean corpuscular volume (MCV)—Adults: 84–96 femtoliters (fL)
How do you evaluate a peripheral blood smear?
Peripheral blood smear can be used for estimation of manual blood counts. With the advent of automated cell counters which are more reliable and accurate, manual differential counts of white blood cells using PBF is gradually fading in routine haematology laboratory practice.
Why a good smear is essential?
From the clinical standpoint, blood smear examination serves 3 important objectives. First, it serves as a quality control tool in verifying the results generated by the automated analyzers. Second, it allows for identification of abnormal/immature/atypical cells, if present.
What are the 4 qualities of a well stained smear?
List 3 characteristics of a well-stained blood smear.
- Stain is not too dark or too pale.
- No stain precipitate is present on the smear.
- RBCs are appropriate color of reddish pink.
How do you evaluate a blood smear?
This is our recommended protocol for blood smear examination: Scan the smear at low magnification, then use a higher magnification to perform a differential leukocyte count, enumerate nucleated RBC, estimate a platelet count, and evaluate red blood cell (RBC), white blood cell (WBC) and platelet morphologic features.
What is PBS study?
A procedure in which a sample of blood is viewed under a microscope to count different circulating blood cells (red blood cells, white blood cells, platelets, etc.) and see whether the cells look normal.
What is the aim of blood smear?
A blood smear is a type of blood test. It looks at the appearance, number, and shape of your red and white blood cells and platelets to see whether they are normal. A blood smear can also detect parasites in your blood.
Which stain is used for peripheral blood smear?
Commonly used stain in our environment is Leishman stain which is composed of polychrome methylene blue (basic component) and eosin (acidic component). May-Grunwald Giemsa or Wright-Giemsa stain can also be used.
What is the principle of blood smear?
A blood smear is made by placing a drop of blood on one end of a slide, and using a spreader slide to disperse the blood over the slide’s length. The aim is to get a region, called a monolayer, where the cells are spaced far enough apart to be counted and differentiated.
What is PS examination?
Peripheral Smear Examination test evaluates the different components of the blood. The smear evaluates the red blood cells, white blood cells, and platelets as well as other abnormalities such as the presence of parasites.
What is the normal ANC count?
A healthy person has an ANC between 2,500 and 6,000. The ANC is found by multiplying the WBC count by the percent of neutrophils in the blood. For instance, if the WBC count is 8,000 and 50% of the WBCs are neutrophils, the ANC is 4,000 (8,000 × 0.50 = 4,000). When the ANC drops below 1,000 it is called neutropenia .
What is the normal size of a peripheral smear?
A well made peripheral smear is thick at one end and progressively thinner at the opposite end. The “zone of morphology” (area of optimal thickness for light microscopic examination) should be at least 2 cm in length. The smear should occupy the central area of the slide and be margin-free at the edges 8.
How to prepare a specimen slide for a blood draw?
Place the slide on a flat surface, and hold the other end between your left thumb and forefinger. 3. With your right hand, place the smooth clean edge of a second (spreader) slide on the specimen slide, just in front of the blood drop.
How do you prepare a smear for a biopsy?
6. SMEAR PREPARATION 1. Place a drop of blood, about 2-3 mm in diameter approximately 1 cm from one end of slide. 2. Place the slide on a flat surface, and hold the other end between your left thumb and forefinger. 3.
What are the disadvantages of peripheral blood smear?
• Cell remnants form dense basophilic masses (the apoptotic bodies) 109. Disadvantages of the Peripheral Blood Smear • Experience is required to make technically adequate smears. • Non-uniform distribution of white blood cells • Larger leukocytes concentrated near edges and lymphocytes scattered throughout.