Table of Contents
What is typing in microbiology?
Molecular typing is a way of identifying specific strains of microorganisms, such as bacteria or viruses, by looking at their genetic material. It is mainly used to pinpoint the source of foodborne outbreaks. It can also be used to identify which microorganisms are: Most virulent and cause serious diseases.
Why is it best to use as many phage types as possible in an phage typing exercise?
Why is it best to use as many phage types as possible in a phage typing exercise? The greater the number of phages, the better chance that the correct phage for the bacterium will be used and the bacterium identified.
When comparing two organisms using DNA hybridization The process relies on what properties of DNA?
When comparing two organisms using DNA hybridization, the process relies on what property of DNA? Accumulations of random mutations provide a relative measure of time elapsed since organisms diverged from a common ancestor.
Why is it best to use as many phage types as possible in an phage typing exercise quizlet?
How does the clearing zone formed in phage typing?
In phage typing, a panel of lytic phages is inoculated on a lawn inoculum of the bacteria under investigation. Phages which are able to set up a lytic infection in that isolate produce a clear zone.
What type of media was used for the plaque assay?
This assay is based on a microbiological method conducted in a plate. Specifically, a confluent monolayer of host cells is infected with the virus at varying dilutions and covered with a semisolid medium, such as agar, to prevent the virus infection from spreading indiscriminately.
What is phage typing in microbiology?
Phage typing. The viruses that infect bacteria are called bacteriophages (“phages” for short) and some of these can only infect a single strain of bacteria. These phages are used to identify different strains of bacteria within a single species. A culture of the strain is grown in the agar and dried.
When was the first phage typing scheme developed?
In 1977, this was extended to 207 types by Anderson at the Enteric Reference Laboratory in London. Since then, phage typing schemes have been developed for Salmonella typhi, Salmonella paratyphi B., Salmonella typhimurium, Shigella sonnei, Staphylococcus aureus, and Escherichia coli to name a few.
What is the size of the culture culture for phage typing?
The culture is 10cm in diameter. Phage typing is a phenotypic method that uses bacteriophages (“phages” for short) for detecting and identifying single strains of bacteria. Phages are viruses that infect bacteria and may lead to bacterial cell lysis.
What is a clear zone in phage typing?
Phages which are able to set up a lytic infection in that isolate produce a clear zone. As the ability to be infected (and lysed) by different phages varies between different strains of bacteria, the pattern of lysis forms the basis of phage typing.