Which chemical is used first in the Diff-Quik method?
The Diff-Quik stain consists of a fixative agent (methanol, blue), solution I (eosinophilic, orange) and solution II (basophilic, blue). Generally, slides are dipped sequentially into each solution 6 times (or left for 10-15 seconds in each solution), followed by a water rinse and drying.
How does Diff Quick work?
RAL Diff-Quik fixative solution It fixes air-dried blood smears. This methanol based solution will stabilize cellular components. Solution I and II are applied individually to the fixed smear to differentially stain specific cellular components.
Which stain is used for FNAC?
Routinely various stains like Haematoxylin and Eosin (H&E), Romanowsky and Pap have been used for staining the FNAC smears. Romanowsky stains are routinely used for staining the blood films and air dried cytological smears [2].
Which chemicals are used during the Gram stain procedure?
Reagents needed for Gram staining include:
- Crystal violet (primary stain) [1]
- Gram’s iodine solution (the mordant) [1]
- Acetone/ethanol (50:50 v:v) (the decolorizer) [1]
- 0.1% basic fuchsin solution (the counterstain) [1]
- Water.
What is Pap stain used for?
Pap stain is a universal stain used for gynecologic and non-gynecologic cytology smear. It is mainly used for oral and cervical cancer screening in asymptomatic population and in the follow up of patients with cancer. Pap test has decreased incidence of cervical cancer by 70% in developed countries.
What is the difference between FNAC and Fnab?
The sampling and biopsy considered together are called fine-needle aspiration biopsy (FNAB) or fine-needle aspiration cytology (FNAC) (the latter to emphasize that any aspiration biopsy involves cytopathology, not histopathology). Fine-needle aspiration biopsies are very safe minor surgical procedures.
What are the various stain used commonly in cytology?
Two of the most commonly used cytological stains are: • Papanicolaou (PAP) stain – Papanicolaou stain is a modi- fied hematoxylin and eosin (H&E) stain and is recom- mended for the staining of alcohol fixed cytology slides 1.
Which reagents are used for staining?
Reagents:
- Crystal violet (primary stain)
- Iodine solution/Gram’s Iodine (mordant that fixes crystal violet to cell wall)
- Decolorizer (e.g. ethanol)
- Safranin (secondary stain)
- Water (preferably in a squirt bottle)
What is the most important reagent in Gram stain method?
crystal violet
The primary stain of the Gram’s method is crystal violet. Crystal violet is sometimes substituted with methylene blue, which is equally effective. The microorganisms that retain the crystal violet-iodine complex appear purple brown under microscopic examination.
What differs EA65 from EA50?
PAP EA65 contains a higher percentage of Green stain component that EA50, it is ofter refered to as ‘ PAP EA65 enhanced green ‘. There are many different formulations for this product across the World. Almost every manufacturer has a slightly different formula.
What is the difference between fine needle aspiration and core biopsy?
While core biopsy obtains a larger tissue sample and provides a degree of architectural information, FNA is considered less invasive and has the advantage of immediate confirmation of adequacy by the attending cytologist.
What is differential Quik III stain kit?
Differential Quik III Stain Kit is similar to the easy to use Differential Quik Stain Kit but with an improved staining quality. It serves as a rapid turnaround staining kit that satisfies many purposes in the cost conscious laboratory environment.
What is the Diff-Quick stain?
The Diff-Quick stain consists of a fixative agent (methanol, blue), solution I (eosinophilic, orange) and solution II (basophilic, blue). Generally, slides are dipped sequentially into each solution 6 times (or left for 10-15 seconds in each solution), followed by a water rinse and drying.
What is the best stain for sperm morphology analysis?
Diff-Quik™ stain for sperm morphology analysis – MICROPTIC Original Diff-Quik™ staining set for sperm morphology analysis with the Sperm Class Analyzer CASA System (SCA). Manufactured by Medion Diagnostics.