Table of Contents
What does SV40 large T antigen do?
The large and small tumor antigens (T antigens) are the major regulatory proteins encoded by SV40. Large T antigen is responsible for both viral and cellular transcriptional regulation, virion assembly, viral DNA replication, and alteration of the cell cycle.
How many proteins are used in SV40 replication?
SV40 DNA replication requires the viral replication origin, which is localized in the NCCR, and only one viral protein, the multifunctional Tag helicase, with the host supplying all other replication factors.
How does SV40 inhibit p53?
Simian virus 40 (SV40) large tumor antigen (T antigen) has been shown to inhibit p53-dependent transcription by preventing p53 from binding to its cognate cis element.
What is SV40 gene?
Abstract. The polyomavirus simian virus 40 (SV40) is a known oncogenic DNA virus which induces primary brain and bone cancers, malignant mesothelioma, and lymphomas in laboratory animals. Persuasive evidence now indicates that SV40 is causing infections in humans today and represents an emerging pathogen.
What does SV40 cause?
The polyomavirus simian virus 40 (SV40) is a known oncogenic DNA virus which induces primary brain and bone cancers, malignant mesothelioma, and lymphomas in laboratory animals. Persuasive evidence now indicates that SV40 is causing infections in humans today and represents an emerging pathogen.
What does the SV40 promoter do?
The early promoter of the simian virus 40 (SV40) has been used as a model eukaryotic promoter for the study of DNA sequence elements and cellular factors that are involved in transcriptional control and initiation.
What is SV40 plasmid?
General description. PSF-SV40 – SV40 PROMOTER PLASMID contains the Simian Virus 40 promoter upstream of the multiple cloning site (MCS) for expression in mammalian cells. Transcription termination is mediated by the SV40 poly-adenylation signals downstream of the MCS.
What is SV40 promoter?
Is SV40 a mammalian promoter?
Human cytomegalovirus major immediate-early (CMV), Rous sarcoma virus (RSV), Homo sapiens ubiquitin C (UBC), simian vacuolating virus 40 (SV40), phosphoglycerate kinase (PGK) and β-globin promoters are the common promoters used in mammalian cells such as CHO cells26,27,28.
What does SV40 stand for?
Simian virus 40. A virus that infects some types of monkeys. It may also infect humans, and was found in some polio vaccines tested in the early 1960s. Although the virus has been shown to cause cancer in laboratory animals, there is no evidence that it causes cancer in people.
Why is SV40 promoter used?
Abstract. The early promoter of the simian virus 40 (SV40) has been used as a model eukaryotic promoter for the study of DNA sequence elements and cellular factors that are involved in transcriptional control and initiation.
What causes polyomavirus?
The human polyomaviruses BKV and JCV are known to cause, respectively, hemorrhagic cystitis in recipients of bone marrow transplantation and progressive multifocal leukoencephalopathy in immunocompromised patients, for example, by HIV infection.