Table of Contents
What is anti GFP antibody?
Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin.
Can GFP detect antibodies?
Rockland anti GFP antibodies are suitable for the detection of native GFP, GFP variants such as S65T-GFP, RS-GFP, YFP and EGFP and most other GFP fusion proteins by ELISA, Flow Cytometry, Immunofluorescent Microscopy, Immunohistochemistry, Immunoprecipitation and western blot.
Is GFP used in immunofluorescence?
Epitope tags and anti-epitope tag antibodies are widely used for purification and detection of recombinant proteins in immunoblotting, immunostaining and immunoprecipitation.
What is Zsgreen?
ZsGreen1 is the brightest commercially available green fluorescent protein—up to 4X brighter than EGFP—and is ideally suited for whole-cell labelling, promoter-reporter studies, or as a transfection control. Living Colors ZsGreen1 is an exceptionally bright green fluorescent protein derived from a Zoanthus sp.
What can GFP be used for?
GFP is used in research across a vast array of biological disciplines and scientists employ GFP for a wide number of functions, including: tagging genes for elucidating their expression or localization profiles, acting as a biosensor or cell marker, studying protein-protein interactions, visualizing promoter activity.
What is a GFP trap antibody?
GFP-Trap consists of an anti-GFP V H H (green circle), derived from camelidae (alpaca, llama, camel, dromedaries, etc.) heavy chain antibodies. The anti-GFP V H H (also termed nanobody or single domain antibody) coupled to agarose beads is the GFP-Trap.
What is anti GFP Nanobody made of?
This nanobody is produced from alpaca with a GFP protein. Magnetic beads Conjugated anti-GFP nanobody, the product is supplied in 10 mM PBS, pH 7.4, and 0.02% (w/v) sodium azide as preservative.
What kind of suspension is used for GFP antibody isotype?
Conjugated antibody isotype, alpaca single domain antibody (nanobody, VHH) Agarose beads Conjugated anti-GFP Nanobody, the product is supplied as a 50% suspension in 10 mM phosphate buffered saline (PBS), pH 7.4, and 0.02% (w/v) sodium azide as preservative.
What is the best monoclonal anti GFP antibody?
The GFP-Trap is the most frequently cited monoclonal anti-GFP antibody, making it the gold standard for immunoprecipitation of GFP-fusion proteins. See publications list on our website here or alternatively,see collection independently validated by citeab.com here.