Table of Contents
What induces FoxP3?
TCR stimulation induces FoxP3 expression in CD4+CD25– human T cells. In addition to being a marker for TR cells, CD25 expression on CD4+ T cells is an indicator of cell activation.
How do you identify Treg cells?
Treg cells can be identified by a combination of different surface markers and the intracellular transcription factor FoxP3. Their main function is the suppression and termination of pro-inflammatory immune responses. They are considered to play a crucial role in several human diseases and mouse models.
What does FOXP3 code for?
The FOXP3 gene provides instructions for producing the forkhead box P3 (FOXP3) protein. The FOXP3 protein attaches (binds) to specific regions of DNA and helps control the activity of genes that are involved in regulating the immune system.
What is the function of Treg cells?
Regulatory T cells (Tregs) are a specialized subpopulation of T cells that act to suppress immune response, thereby maintaining homeostasis and self-tolerance. It has been shown that Tregs are able to inhibit T cell proliferation and cytokine production and play a critical role in preventing autoimmunity.
What is the role of Foxp3?
How are Treg activated?
The activation of Treg cells is antigen-specific, which implies that the suppressive activity of Treg cells is triggered in an antigen-specific fashion. Concerning the target cell, there is evidence that Treg cells may suppress Th cells with different antigen specificities.
What does FOXP3 stain?
The Foxp3 Transcription Factor Staining Buffer Set has been formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.
What does the FOXP3 transcription factor staining buffer set stain?
The Foxp3 Transcription Factor Staining Buffer Set has been formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.
Can I use blimp1-pe antibodies with the ebioscience foxp3/transcription factor staining buffer set?
Can I use the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00) with the Blimp1-PE antibody from another company? Yes, the eBioscience Foxp3/Transcription Factor Staining Buffer Set will allow any antibody to reach an intra-nuclear target to stain cells, including antibodies from other vendors.
Can I perform phosphorylation specific staining and stain for intracellular cytokines simultaneously?
Can I perform phosphorylation specific staining and stain for intracellular cytokines at the same time when using eBioscience phosphorylation-specific antibodies? In theory, if the buffer systems are compatible for the antibodies of interest, this can be done.
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