Table of Contents
What is the 40x objective lens?
Total Magnification of a Compound Light Microscope
| 10x Eyepiece | 25x Eyepiece | |
|---|---|---|
| 4x Scanning Lens | 40x | 100x |
| 10x Low Power Lens | 100x | 250x |
| 40x High Power Lens | 400x | 1000x |
| 100x Oil Immersion Lens | 800x | 2500x |
Which microscope lens is 40x?
High Power Objective Lens (40x) The high-powered objective lens (also called “high dry” lens) is ideal for observing fine details within a specimen sample.
What is objective field number?
The field number is simply the maximum FOV of the objective or eyepiece measured as a diameter in millimeters, so an objective lens with a field number of 18 would have a maximum FOV of 18 mm.
What is the focal length of a 40x objective?
Valumax Objective Lens, 40x, 0.65 NA, 4.4 mm Focal Length.
Is the working distance greater with the 40x or the 10x objective?
Objective Specifications by Magnification
| Magnification | Numerical Aperture | Working Distance (mm) |
|---|---|---|
| 4x | 0.20 | 15.70 |
| 10x | 0.45 | 4.00 |
| 20x | 0.75 | 1.00 |
| 40x | 0.95 | 0.14 |
How do you calculate field number?
Field of View = Field Number (FN) ÷ Objective Magnification When you switch eyepieces or objective lenses, ensure to repeat the FOV calculations with the new inputs on field number and magnifications. For objects that require higher magnifications, convert your measurements from millimeters to micrometers.
What diameter might you expect at 40X and 1000x?
Stage micrometer at 1000x magnification with Olympus Compound Microscope. The diameter of field of view (fov) is 0.184 millimeters (184 micrometers)….
| Objective | Diameter Of Field Of View | Magnification (10x Ocular) |
|---|---|---|
| 4x | 4.0 mm (4.45) | 40x |
| 10x | 2.0 mm (1.78) | 100x |
| 40x | 0.4 mm (0.45) | 400x |
| 100x | 0.2 mm (0.178) | 1000x |
What is the working distance for a 4x objective lens?
Table 1 – Objective Specifications by Magnification
| Achromat Correction | ||
|---|---|---|
| Magnification | Numerical Aperture | Working Distance (mm) |
| 4x | 0.10 | 30.00 |
| 10x | 0.25 | 10.50 |
| 20x | 0.40 | 1.30 |
Where is the field number?
The field number (FN) in microscopy is defined as the diameter of the area in the intermediate image plane that can be observed through the eyepiece. A field number of, e.g., 20 mm indicates that the observed sample area after magnification by the objective lens is restricted to a diameter of 20 mm.
How do you calculate field of view from magnification?
For example, if the eyepiece reads 10x/18, and the magnification of your objective lens is 40, multiply 10 and 40 to get 400. Then divide 18 by 400 to get an FOV diameter of 0.045 millimeters.