Table of Contents
What is photon fluorescence?
Two-photon fluorescence microscopy allows three-dimensional imaging of biological specimens in vivo. Compared with confocal microscopy, it offers the advantages of deeper tissue penetration and less photodamage but has the disadvantage of slightly lower resolution. Nature of Two-photon Absorption.
What is two-photon photoluminescence?
Two-photon photoluminescence (TPPL) is an emission process which can take place when an emitter absorbs two photons of low energy simultaneously and emits a photon of high energy.
What is calcium imaging used for?
Calcium imaging can be used to track the activity of neurons over time and investigate how networks grow or change during learning. This is especially important for the longitudinal study of animal models.
What molecule does GCaMP detect?
It is used in biological research to measure intracellular Ca2+ levels both in vitro and in vivo using virally transfected or transgenic cell and animal lines.
How to pronounce fluorescence?
fluorescence pronunciation with meanings, synonyms, antonyms, translations, sentences and more Which is the right way to pronounce the anachronism? a-nach-ro-nis-m
What is the working principle of fluorescence spectroscopy?
The X-ray tube generates the primary X-radiation.
What does microscopy, fluorescence, multiphoton mean?
Multiphoton fluorescence (2PEF and 3PEF) is a useful mean of imaging the brain in-vivo. Through the (shaved) skull imaging of capillaries and RBCs is published at mice, suggesting greater depth and high resolution from further away than microscopy applications.
What are the types of photon excitation?
Principles of Two-Photon Excitation.